Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PALA8_RS28170 Genome accession   NZ_CP104869
Coordinates   6081496..6082560 (-) Length   354 a.a.
NCBI ID   WP_269973492.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6076496..6087560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA8_RS28145 (PALA8_05600) aroK 6077031..6077549 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA8_RS28150 (PALA8_05601) pilQ 6077561..6079705 (-) 2145 WP_003135767.1 type 4a pilus secretin PilQ Machinery gene
  PALA8_RS28155 (PALA8_05602) pilP 6079759..6080283 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  PALA8_RS28160 (PALA8_05603) pilO 6080280..6080903 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  PALA8_RS28165 (PALA8_05604) pilN 6080900..6081496 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  PALA8_RS28170 (PALA8_05605) pilM 6081496..6082560 (-) 1065 WP_269973492.1 type IV pilus assembly protein PilM Machinery gene
  PALA8_RS28175 (PALA8_05606) - 6082745..6085216 (+) 2472 WP_003135771.1 penicillin-binding protein 1A -
  PALA8_RS28180 (PALA8_05607) - 6085317..6086585 (-) 1269 WP_003095842.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37998.45 Da        Isoelectric Point: 4.5405

>NTDB_id=733009 PALA8_RS28170 WP_269973492.1 6081496..6082560(-) (pilM) [Pseudomonas aeruginosa strain PALA8]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQQGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=733009 PALA8_RS28170 WP_269973492.1 6081496..6082560(-) (pilM) [Pseudomonas aeruginosa strain PALA8]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGATGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCAGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

52.101

100

0.525
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463