Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   P5628_RS08685 Genome accession   NZ_CP120577
Coordinates   1647854..1649800 (+) Length   648 a.a.
NCBI ID   WP_015252109.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO53     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1642854..1654800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS08660 (P5628_08660) def 1643224..1643706 (+) 483 WP_277709581.1 peptide deformylase -
  P5628_RS08665 (P5628_08665) fmt 1643711..1644664 (+) 954 WP_187954238.1 methionyl-tRNA formyltransferase -
  P5628_RS08670 (P5628_08670) rsmB 1644651..1645994 (+) 1344 WP_021479311.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  P5628_RS08675 (P5628_08675) rlmN 1645998..1647089 (+) 1092 WP_021479310.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  P5628_RS08680 (P5628_08680) prpC 1647096..1647860 (+) 765 WP_014479762.1 protein-serine/threonine phosphatase PrpC -
  P5628_RS08685 (P5628_08685) stkP 1647854..1649800 (+) 1947 WP_015252109.1 serine/threonine protein kinase PrkC Regulator
  P5628_RS08690 (P5628_08690) rsgA 1649815..1650711 (+) 897 WP_021479308.1 ribosome small subunit-dependent GTPase A -
  P5628_RS08695 (P5628_08695) rpe 1650716..1651369 (+) 654 WP_277709584.1 ribulose-phosphate 3-epimerase -
  P5628_RS08700 (P5628_08700) thiN 1651442..1652086 (+) 645 WP_277709585.1 thiamine diphosphokinase -
  P5628_RS08705 (P5628_08705) spoVM 1652158..1652238 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  P5628_RS08710 (P5628_08710) rpmB 1652311..1652499 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  P5628_RS08715 (P5628_08715) yloU 1652776..1653138 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71880.56 Da        Isoelectric Point: 4.7871

>NTDB_id=732680 P5628_RS08685 WP_015252109.1 1647854..1649800(+) (stkP) [Bacillus subtilis strain PRO53]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFKHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=732680 P5628_RS08685 WP_015252109.1 1647854..1649800(+) (stkP) [Bacillus subtilis strain PRO53]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTACGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATAGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAGAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTTGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAGACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGTACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAGGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAAACATG
TAACGGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTTGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.517

89.506

0.363
  stkP Streptococcus pneumoniae D39

40.345

89.506

0.361
  stkP Streptococcus pneumoniae R6

40.345

89.506

0.361