Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR87_RS00335 Genome accession   NZ_CP103831
Coordinates   63991..64539 (+) Length   182 a.a.
NCBI ID   WP_279481145.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 1812     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58991..69539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR87_RS00290 (NYR87_00290) lpxK 58992..59972 (+) 981 WP_279481142.1 tetraacyldisaccharide 4'-kinase -
  NYR87_RS00295 (NYR87_00295) - 59974..60141 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR87_RS00305 (NYR87_00305) gltX 60404..61843 (-) 1440 WP_279437278.1 glutamate--tRNA ligase -
  NYR87_RS00330 (NYR87_00330) - 62756..63937 (+) 1182 WP_278225213.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR87_RS00335 (NYR87_00335) pilF 63991..64539 (+) 549 WP_279481145.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR87_RS00340 (NYR87_00340) - 64615..66954 (+) 2340 WP_279481146.1 Tex family protein -
  NYR87_RS00345 (NYR87_00345) - 67225..67512 (+) 288 WP_279481148.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  NYR87_RS00350 (NYR87_00350) - 67512..67934 (+) 423 WP_279481151.1 type II toxin-antitoxin system VapC family toxin -
  NYR87_RS00355 (NYR87_00355) - 68041..69321 (-) 1281 WP_279471384.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20686.32 Da        Isoelectric Point: 6.6304

>NTDB_id=725767 NYR87_RS00335 WP_279481145.1 63991..64539(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 1812]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQSYYHQADSLENIVLCAKQEPNL
VKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725767 NYR87_RS00335 WP_279481145.1 63991..64539(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 1812]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAACAGTTTGAGCAAGCGG
TGCAAAGCGAGCAGTCTTACTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTGTGTGCCAAACAAGAGCCGAATTTG
GTTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44