Detailed information NTG0722865

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: OPY27_RS27350
• Genome accession: NZ_CP118565
• Coordinates: 5848989..5850053 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain PA_HN004
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5843989..5855053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OPY27_RS27325 (OPY27_27315)	aroK	5844524..5845042 (-)	519	WP_003095833.1	shikimate kinase AroK	-
OPY27_RS27330 (OPY27_27320)	pilQ	5845054..5847198 (-)	2145	WP_003135767.1	type 4a pilus secretin PilQ	Machinery gene
OPY27_RS27335 (OPY27_27325)	comL/pilP	5847252..5847776 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
OPY27_RS27340 (OPY27_27330)	pilO	5847773..5848396 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
OPY27_RS27345 (OPY27_27335)	pilN	5848393..5848989 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
OPY27_RS27350 (OPY27_27340)	pilM	5848989..5850053 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
OPY27_RS27355 (OPY27_27345)	-	5850238..5852706 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
OPY27_RS27360 (OPY27_27350)	-	5852811..5854079 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=722865 OPY27_RS27350 WP_003110888.1 5848989..5850053(-) (pilM) [Pseudomonas aeruginosa strain PA_HN004]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=722865 OPY27_RS27350 WP_003110888.1 5848989..5850053(-) (pilM) [Pseudomonas aeruginosa strain PA_HN004]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466