Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL334_RS11530 Genome accession   NZ_AP019192
Coordinates   2123769..2125067 (-) Length   432 a.a.
NCBI ID   WP_000867590.1    Uniprot ID   A0AA95DCE6
Organism   Streptococcus pneumoniae strain ASP0581     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2118769..2130067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL334_RS11510 (ASP0581_21260) rplI 2119924..2120376 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  EL334_RS11515 (ASP0581_21270) - 2120373..2122346 (-) 1974 WP_000715129.1 DHH family phosphoesterase -
  EL334_RS11520 (ASP0581_21280) hpf 2122482..2123030 (-) 549 WP_033705088.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL334_RS11525 (ASP0581_21290) comFC/cflB 2123110..2123772 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  EL334_RS11530 (ASP0581_21300) comFA/cflA 2123769..2125067 (-) 1299 WP_000867590.1 DEAD/DEAH box helicase Machinery gene
  EL334_RS11535 (ASP0581_21310) - 2125123..2125758 (+) 636 WP_000395739.1 YigZ family protein -
  EL334_RS11540 (ASP0581_21320) cysK 2126043..2126963 (+) 921 WP_033705087.1 cysteine synthase A -
  EL334_RS11550 (ASP0581_21330) - 2127038..2127576 (-) 539 Protein_2127 IS66 family transposase -
  EL334_RS11555 - 2127549..2127857 (+) 309 Protein_2128 transposase -
  EL334_RS11560 (ASP0581_21350) tnpB 2127880..2128230 (-) 351 WP_033705086.1 IS66 family insertion sequence element accessory protein TnpB -
  EL334_RS11565 (ASP0581_21360) tsf 2128477..2129517 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49574.61 Da        Isoelectric Point: 8.5992

>NTDB_id=72181 EL334_RS11530 WP_000867590.1 2123769..2125067(-) (comFA/cflA) [Streptococcus pneumoniae strain ASP0581]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=72181 EL334_RS11530 WP_000867590.1 2123769..2125067(-) (comFA/cflA) [Streptococcus pneumoniae strain ASP0581]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTATTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGAATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.696

94.444

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment