Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NM966_RS05335 Genome accession   NZ_CP101506
Coordinates   1048747..1052256 (+) Length   1169 a.a.
NCBI ID   WP_046526635.1    Uniprot ID   -
Organism   Bacillus altitudinis strain B12     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1043747..1057256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM966_RS05305 (NM966_05295) lepB 1044255..1044767 (+) 513 WP_255392064.1 signal peptidase I -
  NM966_RS05310 (NM966_05300) - 1044803..1045945 (-) 1143 WP_165379353.1 AbrB family transcriptional regulator -
  NM966_RS05315 (NM966_05305) - 1046220..1046642 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  NM966_RS05320 (NM966_05310) - 1046635..1047474 (+) 840 WP_017360221.1 M56 family metallopeptidase -
  NM966_RS05325 (NM966_05315) - 1047532..1047942 (+) 411 WP_236810361.1 DoxX family protein -
  NM966_RS05330 (NM966_05320) - 1048134..1048580 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  NM966_RS05335 (NM966_05325) addB 1048747..1052256 (+) 3510 WP_046526635.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NM966_RS05340 (NM966_05330) addA 1052234..1055938 (+) 3705 WP_191218908.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NM966_RS05345 (NM966_05335) sbcD 1055964..1057139 (+) 1176 WP_025207109.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134717.72 Da        Isoelectric Point: 5.3149

>NTDB_id=710278 NM966_RS05335 WP_046526635.1 1048747..1052256(+) (addB) [Bacillus altitudinis strain B12]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVRGEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMIDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYVSYSASDTEGRSLLPSTLIKYYEELMPNHQQALYVLDPEQLEDQQQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNRDVSKKIMSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLAEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=710278 NM966_RS05335 WP_046526635.1 1048747..1052256(+) (addB) [Bacillus altitudinis strain B12]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTGCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGGATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGATTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAAATCTCGGCATCCAGTATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGGGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCAGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGCTGGCCAA
AGAGTGTGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTAAGAGGGGAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACATGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGCGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCTAAAAACAAAGCAAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGATTTCATTATCGCCGCT
TCCAATCACTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAAGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGCTTGAGGCAGAAGAAGCTGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTGTGGGATGCTGTCATTCAATTGATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTA
TCATTTCCATTATTTCAACAGATGATAGATACAGGACTGTCCTCTCTCAAGTTTGCTTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGAGTTA
TACCTGCACGGCCCTCTGATGAAAGTGTATTGTCTGAAGACGACCGGGAATGGTTAAAACGGGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATATATCAAGCGCTGTCCAGTCCATCGCACTACCTGTATGT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTCTTTTGCCTTCGACCCTCATCAAGTATTATGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGAAGATCAGCAGCAATTAAAATTTGTAACAAACGAGCAT
GTCTCGTTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACCGCGACGTATCAAAAAAAATCATGTCCAGCTTGTTCTTTACAAATAGAGCGAAAC
GTTTAAAGCCGAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGTTAAAACTAAAAGATCGACAATTTTACAAATTAGAAGCACCTGATATCGG
CCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCATGCAATCGAACAGCTGGCTCCACGTCTTCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTCCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCCTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAAGCGACACCGGCAGGAATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCGCCTATTCCGCTCGCAGAAGATGCGATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAAATAAACCCATATAAATTAAAGGATCAAACCCCT
TGCCGTTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACCTCTGA
GAAGGATGATGTCATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.667

99.316

0.662