Detailed information NTG0709668

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: PIB33_RS01670
• Genome accession: NZ_CP116391
• Coordinates: 303990..305111 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain GXD-20
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 298990..310111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PIB33_RS01645 (PIB33_01645)	ycdA	298998..300062 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
PIB33_RS01650 (PIB33_01650)	-	300085..300216 (-)	132	WP_120363245.1	hypothetical protein	-
PIB33_RS01655 (PIB33_01655)	ycdB	300390..301808 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
PIB33_RS01660 (PIB33_01660)	ycdC	301995..303356 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
PIB33_RS01665 (PIB33_01665)	cwlK	303364..303867 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
PIB33_RS01670 (PIB33_01670)	rapF	303990..305111 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
PIB33_RS01675 (PIB33_01675)	ycdF	305218..305994 (+)	777	WP_019712418.1	SDR family oxidoreductase	-
PIB33_RS01680 (PIB33_01680)	ycdG	306019..307704 (+)	1686	WP_003234736.1	alpha-glucosidase	-
PIB33_RS01685 (PIB33_01685)	znuA	307892..308851 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
PIB33_RS01690 (PIB33_01690)	znuC	308907..309602 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=709668 PIB33_RS01670 WP_003246478.1 303990..305111(+) (rapF) [Bacillus subtilis strain GXD-20]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=709668 PIB33_RS01670 WP_003246478.1 303990..305111(+) (rapF) [Bacillus subtilis strain GXD-20]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGACACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405