Detailed information NTG0704240

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NKX97_RS21045
• Genome accession: NZ_CP100312
• Coordinates: 4290633..4291433 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 1020
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4285633..4296433

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NKX97_RS21010 (NKX97_20995)	gspH	4285789..4286304 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
NKX97_RS21015 (NKX97_21000)	gspI	4286301..4286666 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NKX97_RS21020 (NKX97_21005)	gspJ	4286663..4287259 (+)	597	WP_004191953.1	type II secretion system minor pseudopilin GspJ	-
NKX97_RS21025 (NKX97_21010)	gspK	4287256..4288242 (+)	987	WP_023307453.1	type II secretion system minor pseudopilin GspK	-
NKX97_RS21030 (NKX97_21015)	gspL	4288229..4289422 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
NKX97_RS21035 (NKX97_21020)	-	4289415..4289900 (+)	486	WP_023307455.1	type II secretion system protein M	-
NKX97_RS21040 (NKX97_21025)	-	4289890..4290636 (+)	747	WP_004145897.1	type II secretion system protein N	-
NKX97_RS21045 (NKX97_21030)	pilD	4290633..4291433 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
NKX97_RS21050 (NKX97_21035)	hrpB	4291481..4293910 (-)	2430	WP_004191962.1	ATP-dependent helicase HrpB	-
NKX97_RS21055 (NKX97_21040)	thpR	4293983..4294519 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
NKX97_RS21060 (NKX97_21045)	sfsA	4294519..4295235 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NKX97_RS21065 (NKX97_21050)	dksA	4295398..4295853 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=704240 NKX97_RS21045 WP_004191960.1 4290633..4291433(+) (pilD) [Klebsiella pneumoniae strain 1020]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=704240 NKX97_RS21045 WP_004191960.1 4290633..4291433(+) (pilD) [Klebsiella pneumoniae strain 1020]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421