Detailed information NTG0070416

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: H7675_RS00335
• Genome accession: NZ_AP023389
• Coordinates: 54024..55022 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain KUN-0012590
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49024..60022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7675_RS00320 (KUN2590_00290)	-	50028..51446 (+)	1419	WP_023612101.1	hypothetical protein	-
H7675_RS00325 (KUN2590_00300)	purB	51466..52758 (+)	1293	WP_023612104.1	adenylosuccinate lyase	-
H7675_RS00330 (KUN2590_00310)	rgg4	52888..53798 (+)	911	Protein_32	transcriptional regulator Rgg4/ComR	-
H7675_RS09505	comS	53882..53977 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
H7675_RS00335 (KUN2590_00330)	ruvB	54024..55022 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
H7675_RS00340 (KUN2590_00340)	-	55160..55598 (+)	439	Protein_35	low molecular weight protein-tyrosine-phosphatase	-
H7675_RS00345 (KUN2590_00350)	-	55622..56023 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
H7675_RS00350 (KUN2590_00360)	-	56020..57795 (+)	1776	WP_014407199.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=70416 H7675_RS00335 WP_002986679.1 54024..55022(+) (ruvB) [Streptococcus pyogenes strain KUN-0012590]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=70416 H7675_RS00335 WP_002986679.1 54024..55022(+) (ruvB) [Streptococcus pyogenes strain KUN-0012590]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGATTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAAAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473