Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP34_RS27695 Genome accession   NZ_CP115299
Coordinates   5944569..5945192 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5939569..5950192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP34_RS27680 (PCP34_27665) - 5940256..5942727 (-) 2472 WP_023518163.1 penicillin-binding protein 1A -
  PCP34_RS27685 (PCP34_27670) pilM 5942912..5943976 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP34_RS27690 (PCP34_27675) pilN 5943976..5944572 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP34_RS27695 (PCP34_27680) pilO 5944569..5945192 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP34_RS27700 (PCP34_27685) comL/pilP 5945189..5945713 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP34_RS27705 (PCP34_27690) pilQ 5945767..5947911 (+) 2145 WP_015503770.1 type 4a pilus secretin PilQ Machinery gene
  PCP34_RS27710 (PCP34_27695) aroK 5947923..5948441 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PCP34_RS27715 (PCP34_27700) aroB 5948491..5949597 (+) 1107 WP_003112148.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=703071 PCP34_RS27695 WP_003103268.1 5944569..5945192(+) (pilO) [Pseudomonas aeruginosa strain 20]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=703071 PCP34_RS27695 WP_003103268.1 5944569..5945192(+) (pilO) [Pseudomonas aeruginosa strain 20]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401