Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP32_RS17995 Genome accession   NZ_CP115285
Coordinates   3864509..3865132 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3859509..3870132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP32_RS17975 (PCP32_17970) aroB 3860104..3861210 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  PCP32_RS17980 (PCP32_17975) aroK 3861260..3861778 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PCP32_RS17985 (PCP32_17980) pilQ 3861790..3863934 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  PCP32_RS17990 (PCP32_17985) comL/pilP 3863988..3864512 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP32_RS17995 (PCP32_17990) pilO 3864509..3865132 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP32_RS18000 (PCP32_17995) pilN 3865129..3865725 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP32_RS18005 (PCP32_18000) pilM 3865725..3866789 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP32_RS18010 (PCP32_18005) - 3866974..3869445 (+) 2472 WP_058152670.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=701894 PCP32_RS17995 WP_003103268.1 3864509..3865132(-) (pilO) [Pseudomonas aeruginosa strain F007]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=701894 PCP32_RS17995 WP_003103268.1 3864509..3865132(-) (pilO) [Pseudomonas aeruginosa strain F007]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401