Detailed information NTG0700557

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: PCP29_RS10000
• Genome accession: NZ_CP115269
• Coordinates: 2118200..2118673 (+)
• Length: 157 a.a.
• NCBI ID: WP_015503553.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F020
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2113200..2123673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP29_RS09975 (PCP29_09975)	yacG	2113368..2113568 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PCP29_RS09980 (PCP29_09980)	coaE	2113565..2114176 (-)	612	WP_003112838.1	dephospho-CoA kinase	-
PCP29_RS09985 (PCP29_09985)	pilD	2114173..2115045 (-)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP29_RS09990 (PCP29_09990)	pilC	2115048..2116265 (-)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
PCP29_RS09995 (PCP29_09995)	pilB	2116269..2117969 (-)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP29_RS10000 (PCP29_10000)	pilE	2118200..2118673 (+)	474	WP_015503553.1	pilin	Machinery gene
PCP29_RS10010 (PCP29_10010)	-	2118904..2119146 (-)	243	WP_023082027.1	hypothetical protein	-
PCP29_RS10015 (PCP29_10015)	-	2120175..2120434 (-)	260	Protein_1969	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
PCP29_RS10020 (PCP29_10020)	fliR	2120437..2121219 (-)	783	Protein_1970	flagellar biosynthetic protein FliR	-
PCP29_RS10025 (PCP29_10025)	fliQ	2121219..2121488 (-)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
PCP29_RS10030 (PCP29_10030)	fliP	2121533..2122288 (-)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-
PCP29_RS10035 (PCP29_10035)	-	2122568..2123230 (-)	663	WP_033992423.1	DUF3218 family protein	-

Sequence

Protein

• Length: 157 a.a.
• Molecular weight: 16090.45 Da
• Isoelectric Point: 8.4684

>NTDB_id=700557 PCP29_RS10000 WP_015503553.1 2118200..2118673(+) (pilE) [Pseudomonas aeruginosa strain F020]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide

• Length: 474 bp

>NTDB_id=700557 PCP29_RS10000 WP_015503553.1 2118200..2118673(+) (pilE) [Pseudomonas aeruginosa strain F020]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-150)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	42.197	100	0.465
comP	Acinetobacter baylyi ADP1	47.059	97.452	0.459
pilE	Neisseria gonorrhoeae MS11	42.515	100	0.452
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	41.176	100	0.446
pilA/pilA1	Eikenella corrodens VA1	41.975	100	0.433
pilA2	Legionella pneumophila str. Paris	45.578	93.631	0.427
pilA2	Legionella pneumophila strain ERS1305867	44.218	93.631	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	39.394	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.333	95.541	0.414
pilA	Pseudomonas aeruginosa PAK	41.722	96.178	0.401
pilA	Acinetobacter nosocomialis M2	55.357	71.338	0.395