Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   OTU47_RS03450 Genome accession   NZ_CP113115
Coordinates   673259..674275 (+) Length   338 a.a.
NCBI ID   WP_012512625.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NP2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 668259..679275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU47_RS03430 (OTU47_03430) nagA 669406..670557 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  OTU47_RS03435 (OTU47_03435) - 670720..671778 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  OTU47_RS03440 (OTU47_03440) - 671929..672294 (+) 366 WP_000286415.1 DUF1033 family protein -
  OTU47_RS03445 (OTU47_03445) comGA/cglA/cilD 672370..673311 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  OTU47_RS03450 (OTU47_03450) comGB/cglB 673259..674275 (+) 1017 WP_012512625.1 competence type IV pilus assembly protein ComGB Machinery gene
  OTU47_RS03460 (OTU47_03460) - 674357..675802 (-) 1446 WP_267748066.1 recombinase family protein -
  OTU47_RS03465 (OTU47_03465) - 675976..676176 (-) 201 WP_000064302.1 hypothetical protein -
  OTU47_RS03470 (OTU47_03470) - 676178..676966 (-) 789 WP_050116194.1 helix-turn-helix domain-containing protein -
  OTU47_RS03475 (OTU47_03475) - 677121..677396 (+) 276 WP_001094375.1 hypothetical protein -
  OTU47_RS03480 (OTU47_03480) - 677471..677638 (+) 168 WP_267748061.1 hypothetical protein -
  OTU47_RS03485 (OTU47_03485) - 677629..678078 (-) 450 WP_001058908.1 hypothetical protein -
  OTU47_RS03490 (OTU47_03490) - 678277..678567 (+) 291 WP_001815531.1 hypothetical protein -
  OTU47_RS03495 (OTU47_03495) - 678759..678947 (+) 189 WP_001161207.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38524.56 Da        Isoelectric Point: 9.6162

>NTDB_id=689775 OTU47_RS03450 WP_012512625.1 673259..674275(+) (comGB/cglB) [Streptococcus pneumoniae strain NP2]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LVMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=689775 OTU47_RS03450 WP_012512625.1 673259..674275(+) (comGB/cglB) [Streptococcus pneumoniae strain NP2]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGTCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comGB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comGB Streptococcus mutans UA140

57.595

93.491

0.538
  comGB Streptococcus mutans UA159

57.595

93.491

0.538
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503