Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   OTU47_RS03445 Genome accession   NZ_CP113115
Coordinates   672370..673311 (+) Length   313 a.a.
NCBI ID   WP_000249552.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NP2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 667370..678311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU47_RS03425 (OTU47_03425) - 667436..669253 (+) 1818 WP_001220922.1 acyltransferase family protein -
  OTU47_RS03430 (OTU47_03430) nagA 669406..670557 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  OTU47_RS03435 (OTU47_03435) - 670720..671778 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  OTU47_RS03440 (OTU47_03440) - 671929..672294 (+) 366 WP_000286415.1 DUF1033 family protein -
  OTU47_RS03445 (OTU47_03445) comGA/cglA/cilD 672370..673311 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  OTU47_RS03450 (OTU47_03450) comGB/cglB 673259..674275 (+) 1017 WP_012512625.1 competence type IV pilus assembly protein ComGB Machinery gene
  OTU47_RS03460 (OTU47_03460) - 674357..675802 (-) 1446 WP_267748066.1 recombinase family protein -
  OTU47_RS03465 (OTU47_03465) - 675976..676176 (-) 201 WP_000064302.1 hypothetical protein -
  OTU47_RS03470 (OTU47_03470) - 676178..676966 (-) 789 WP_050116194.1 helix-turn-helix domain-containing protein -
  OTU47_RS03475 (OTU47_03475) - 677121..677396 (+) 276 WP_001094375.1 hypothetical protein -
  OTU47_RS03480 (OTU47_03480) - 677471..677638 (+) 168 WP_267748061.1 hypothetical protein -
  OTU47_RS03485 (OTU47_03485) - 677629..678078 (-) 450 WP_001058908.1 hypothetical protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35587.57 Da        Isoelectric Point: 6.0083

>NTDB_id=689774 OTU47_RS03445 WP_000249552.1 672370..673311(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NP2]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=689774 OTU47_RS03445 WP_000249552.1 672370..673311(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NP2]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGTCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACATCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comGA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comGA Streptococcus mutans UA159

66.238

99.361

0.658
  comGA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364