Detailed information NTG0685056

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: OOZ27_RS02035
• Genome accession: NZ_CP110634
• Coordinates: 357600..358721 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain MG-1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 352600..363721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OOZ27_RS02010 (OOZ27_02010)	ycdA	352608..353672 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
OOZ27_RS02015 (OOZ27_02015)	-	353695..353826 (-)	132	WP_120363245.1	hypothetical protein	-
OOZ27_RS02020 (OOZ27_02020)	ycdB	354000..355418 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
OOZ27_RS02025 (OOZ27_02025)	ycdC	355605..356966 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
OOZ27_RS02030 (OOZ27_02030)	cwlK	356974..357477 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
OOZ27_RS02035 (OOZ27_02035)	rapF	357600..358721 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
OOZ27_RS02040 (OOZ27_02040)	ycdF	358828..359604 (+)	777	WP_019712418.1	SDR family oxidoreductase	-
OOZ27_RS02045 (OOZ27_02045)	ycdG	359629..361314 (+)	1686	WP_003234736.1	glycoside hydrolase family 13 protein	-
OOZ27_RS02050 (OOZ27_02050)	znuA	361502..362461 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
OOZ27_RS02055 (OOZ27_02055)	znuC	362517..363212 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=685056 OOZ27_RS02035 WP_003246478.1 357600..358721(+) (rapF) [Bacillus subtilis strain MG-1]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=685056 OOZ27_RS02035 WP_003246478.1 357600..358721(+) (rapF) [Bacillus subtilis strain MG-1]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGACACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405