Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PALA36_RS25985 Genome accession   NZ_CP110348
Coordinates   5527368..5527907 (-) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5522368..5532907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA36_RS32265 (PALA36_05126) - 5522960..5523511 (-) 552 Protein_5139 RHS repeat protein -
  PALA36_RS25965 - 5524509..5524661 (-) 153 Protein_5140 hypothetical protein -
  PALA36_RS25970 (PALA36_05127) - 5526121..5526363 (+) 243 WP_023109353.1 hypothetical protein -
  PALA36_RS25980 (PALA36_05129) - 5526606..5527304 (-) 699 WP_223677410.1 hypothetical protein -
  PALA36_RS25985 pilE 5527368..5527907 (-) 540 WP_033938465.1 pilin Machinery gene
  PALA36_RS25990 (PALA36_05130) pilB 5528136..5529839 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA36_RS25995 (PALA36_05131) pilC 5529843..5531060 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PALA36_RS26000 (PALA36_05132) pilD 5531063..5531935 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA36_RS26005 (PALA36_05133) coaE 5531932..5532543 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA36_RS26010 (PALA36_05134) yacG 5532540..5532740 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=684094 PALA36_RS25985 WP_033938465.1 5527368..5527907(-) (pilE) [Pseudomonas aeruginosa strain PALA36]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=684094 PALA36_RS25985 WP_033938465.1 5527368..5527907(-) (pilE) [Pseudomonas aeruginosa strain PALA36]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363