Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   JLH08_RS09865 Genome accession   NZ_AP022540
Coordinates   2095776..2096786 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain THO-010     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2090776..2101786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLH08_RS09835 (THOESC010_19230) nudB 2092500..2092952 (+) 453 WP_032154078.1 dihydroneopterin triphosphate diphosphatase -
  JLH08_RS09840 (THOESC010_19240) yebC 2092981..2093721 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  JLH08_RS09845 (THOESC010_19250) ruvC 2093756..2094277 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  JLH08_RS09850 (THOESC010_19260) yebB 2094279..2094881 (-) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  JLH08_RS09855 yobI 2094952..2095017 (+) 66 WP_010723105.1 stress response small protein YobI -
  JLH08_RS09860 (THOESC010_19270) ruvA 2095156..2095767 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  JLH08_RS09865 (THOESC010_19280) ruvB 2095776..2096786 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  JLH08_RS09870 (THOESC010_19290) znuB 2097030..2097815 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  JLH08_RS09875 (THOESC010_19300) znuC 2097812..2098567 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  JLH08_RS09880 (THOESC010_19310) znuA 2098646..2099578 (+) 933 WP_040235277.1 zinc ABC transporter substrate-binding protein ZnuA -
  JLH08_RS09885 (THOESC010_19320) mepM 2099594..2100916 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=68264 JLH08_RS09865 WP_000568519.1 2095776..2096786(+) (ruvB) [Escherichia coli strain THO-010]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=68264 JLH08_RS09865 WP_000568519.1 2095776..2096786(+) (ruvB) [Escherichia coli strain THO-010]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACTACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAGCCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment