Detailed information NTG0067916

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LAB08_RS12815
• Genome accession: NZ_AP017423
• Coordinates: 2855991..2857082 (+)
• Length: 363 a.a.
• NCBI ID: WP_096511855.1
• Uniprot ID: -
• Organism: Pseudomonas izuensis strain LAB-08
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2850991..2862082

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LAB08_RS12800 (LAB08_R25550)	-	2851858..2852790 (+)	933	WP_096511857.1	sugar kinase	-
LAB08_RS12805 (LAB08_R25560)	-	2852995..2853270 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
LAB08_RS12810 (LAB08_R25570)	-	2853335..2855872 (-)	2538	WP_096511856.1	PAS domain S-box protein	-
LAB08_RS12815 (LAB08_R25580)	pilU	2855991..2857082 (+)	1092	WP_096511855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LAB08_RS12820 (LAB08_R25590)	-	2857197..2859152 (-)	1956	WP_096511854.1	acetoacetate--CoA ligase	-
LAB08_RS12825 (LAB08_R25600)	hbdH	2859271..2860044 (-)	774	WP_096511853.1	3-hydroxybutyrate dehydrogenase	-
LAB08_RS12830 (LAB08_R25610)	-	2860052..2861443 (-)	1392	WP_096511852.1	GntP family permease	-

Sequence

Protein

• Length: 363 a.a.
• Molecular weight: 40572.42 Da
• Isoelectric Point: 6.5084

>NTDB_id=67916 LAB08_RS12815 WP_096511855.1 2855991..2857082(+) (pilU) [Pseudomonas izuensis strain LAB-08]
MEIDALLKRLASQDGSDLYLSTGAPPSARFDGVLTPLADQSFKPGEIAAIAASIMDAEQRREFDRELEMNLAISRAGVGR
FRVNIFKQRNDVSMVVRNVKLDIPRFEDLMLPSVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSRGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHSALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERRRQLLHDLGNNLKACVSQRLVRSRDGQRRVAVEVMLGTPTVGDLIRLNQFSELKGVMEKSAALGMQTFDGAL
FELVAEGVVEEDEALKHADSLNNLRLRLKLHAEVSPGDWGLMD

Nucleotide

• Length: 1092 bp

>NTDB_id=67916 LAB08_RS12815 WP_096511855.1 2855991..2857082(+) (pilU) [Pseudomonas izuensis strain LAB-08]
ATGGAAATCGATGCGTTGTTAAAACGCCTGGCCAGCCAGGATGGCTCCGACCTTTATCTGTCCACCGGTGCGCCACCCAG
TGCGCGGTTCGACGGTGTTCTCACGCCCCTGGCGGACCAATCGTTCAAGCCGGGGGAGATTGCGGCGATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCGGGAGTTCGACCGGGAGCTGGAAATGAACCTGGCGATCTCCCGGGCGGGGGTTGGACGC
TTTCGGGTCAATATCTTCAAGCAGCGCAACGATGTGTCCATGGTGGTGCGCAACGTCAAGCTCGACATACCGCGTTTCGA
AGACCTGATGTTGCCGTCGGTATTGCTTGAAACGGTGATGCTCAAGCAAGGCCTGATGCTCTTCGTCGGCGCCACCGACT
CGGGCAAGTCCACGTCGCTGGCGGCCCTGATCGATTATCGCAATCGTCACAGCCGTGGTCATATCGTCACCATCGAGGAC
CCGATCGAATACATCCACCGGCACAAACAATCGATCATCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTCCATAG
TGCGTTGAAAAACACCCTGCGTCAGGCGCCGGATGTGGTGCTGATCGGTGAAATCCGCGACCGCGAGACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGACATCTGGTGATCTCCACGCTGCACGCCCATAACGCCAACCAGGCGCTTGACCGCATC
ATCAACTTCTTCCCCGAAGAGCGCCGCCGGCAATTGCTCCATGACCTGGGCAATAACCTCAAGGCCTGCGTGTCTCAACG
CCTGGTACGCAGCCGGGATGGGCAACGACGGGTGGCGGTGGAGGTGATGCTCGGGACACCGACCGTTGGCGACCTGATCC
GGCTTAACCAGTTTTCCGAACTCAAGGGTGTGATGGAAAAATCAGCGGCGTTGGGGATGCAGACGTTTGACGGAGCGTTG
TTCGAGTTGGTAGCAGAAGGCGTGGTTGAAGAGGACGAAGCGTTGAAGCATGCGGATTCGCTGAATAATTTGCGCTTGCG
GTTGAAACTGCACGCTGAAGTATCACCGGGCGACTGGGGCCTCATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.648	97.796	0.534
pilU	Acinetobacter baylyi ADP1	52.101	98.347	0.512
pilU	Vibrio cholerae strain A1552	49.429	96.419	0.477
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	91.736	0.375
pilT	Vibrio cholerae strain A1552	40.841	91.736	0.375
pilT	Pseudomonas stutzeri DSM 10701	39.535	94.766	0.375
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.823	93.388	0.372
pilT	Legionella pneumophila strain Lp02	39.181	94.215	0.369
pilT	Legionella pneumophila strain ERS1305867	39.181	94.215	0.369
pilT	Pseudomonas aeruginosa PAK	38.953	94.766	0.369
pilT	Neisseria gonorrhoeae MS11	38.416	93.939	0.361
pilT	Neisseria meningitidis 8013	38.416	93.939	0.361