Detailed information NTG0067008

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: TAO_RS05830
• Genome accession: NZ_AP014836
• Coordinates: 1212749..1213930 (+)
• Length: 393 a.a.
• NCBI ID: WP_096527041.1
• Uniprot ID: A0A1Q2SMY6
• Organism: Candidatus Nitrosoglobus terrae strain TAO100
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1207749..1218930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TAO_RS05815 (TAO_1123)	-	1208600..1210807 (+)	2208	WP_096527039.1	TonB-dependent siderophore receptor	-
TAO_RS05820 (TAO_1124)	gpmA	1210894..1211577 (-)	684	WP_096527775.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
TAO_RS05825 (TAO_1125)	-	1211801..1212550 (+)	750	WP_096527040.1	molybdopterin-binding protein	-
TAO_RS05830 (TAO_1126)	pilU	1212749..1213930 (+)	1182	WP_096527041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
TAO_RS05835 (TAO_1127)	-	1214120..1214446 (+)	327	WP_096527042.1	multidrug efflux SMR transporter	-
TAO_RS09760 (TAO_1128)	-	1214533..1214976 (+)	444	WP_197702523.1	hypothetical protein	-
TAO_RS05845 (TAO_1129)	-	1215025..1215468 (-)	444	WP_096527043.1	YcgN family cysteine cluster protein	-
TAO_RS05850 (TAO_1130)	-	1215586..1215870 (-)	285	WP_096527044.1	hypothetical protein	-
TAO_RS05855 (TAO_1131)	-	1216076..1217245 (-)	1170	WP_096527045.1	ChaN family lipoprotein	-
TAO_RS05860 (TAO_1132)	hslO	1217622..1218491 (+)	870	WP_096527046.1	Hsp33 family molecular chaperone HslO	-

Sequence

Protein

• Length: 393 a.a.
• Molecular weight: 44437.09 Da
• Isoelectric Point: 7.0294

>NTDB_id=67008 TAO_RS05830 WP_096527041.1 1212749..1213930(+) (pilU) [Candidatus Nitrosoglobus terrae strain TAO100]
MEFKDYLTIFTIYDGSDIYLSAGAPPCAKFHGHLKPIENKTMTPDEIKEIAYSLMNQEQIAEFERKLEMNLAISKPGIGR
FRVNIFKQRSTISMVIRNIKTEIPDIDHLGLPSILKEIALAKNGLILFIGATGSGKSTSLAAFIDYRNRNSAGHIITIED
PIEYLYTHKKSIVSQREVGVDTNNYEDALKNALRQAPDVILIGEIRDRNTMEHALTFSETGHLCLSTLHANNANQALDRI
INFFPEDRRSQILLDLSLNLRAFISQRLIPTVDGKRIAAIEILLDTPLVRDLIQRGEIYAIKEIMEKSENLGMQTFDNAL
YRLYKAGHIRIEEALHYADSPNNLRLKINLSNKTLDQINEATKKELSLDKIAEKSIKTSKSDDLTLNLVKEGK

Nucleotide

• Length: 1182 bp

>NTDB_id=67008 TAO_RS05830 WP_096527041.1 1212749..1213930(+) (pilU) [Candidatus Nitrosoglobus terrae strain TAO100]
ATGGAATTTAAAGACTATCTAACAATATTTACGATTTATGATGGCTCCGATATTTATCTAAGCGCTGGAGCACCTCCTTG
CGCTAAATTTCATGGTCATTTAAAGCCTATTGAAAACAAAACCATGACTCCTGATGAAATCAAAGAAATCGCTTATAGCC
TAATGAATCAAGAGCAAATTGCTGAATTTGAGCGCAAGCTAGAAATGAATCTCGCTATTTCTAAACCAGGTATTGGGCGC
TTTCGAGTGAATATCTTTAAACAACGTAGCACCATCTCAATGGTGATCCGAAATATTAAAACTGAAATTCCAGATATTGA
TCATCTTGGTTTACCCTCAATATTAAAAGAAATTGCGTTGGCTAAAAATGGGCTTATCCTATTTATAGGGGCTACTGGAT
CTGGTAAATCAACCTCCCTTGCGGCCTTTATTGATTACCGTAATCGTAATAGTGCCGGCCACATCATCACCATTGAAGAT
CCCATTGAGTATTTATATACCCATAAAAAATCCATCGTCAGCCAACGTGAAGTGGGGGTGGATACCAATAACTATGAAGA
TGCCCTTAAAAATGCCCTTCGCCAAGCCCCAGATGTTATTCTTATCGGAGAAATCCGCGATCGGAACACGATGGAACATG
CGCTTACTTTTTCCGAAACAGGCCACTTATGCCTATCTACCTTACATGCTAATAATGCTAATCAAGCTCTTGACCGAATT
ATTAATTTTTTTCCTGAAGATCGTCGATCACAAATTTTATTGGATTTATCACTAAACTTACGTGCTTTTATTTCTCAACG
CCTTATTCCTACTGTTGATGGTAAACGTATTGCAGCCATTGAAATCCTACTCGATACCCCGCTAGTTCGCGATCTTATCC
AACGAGGTGAAATTTATGCCATTAAAGAAATCATGGAAAAGTCAGAAAATTTAGGGATGCAAACCTTTGATAACGCTTTA
TATAGGCTATATAAGGCAGGGCATATCCGCATTGAAGAGGCGCTACATTATGCCGACTCTCCCAATAATTTGCGGCTTAA
AATTAACTTATCCAATAAAACGTTAGATCAGATTAATGAGGCCACTAAAAAAGAACTTTCTCTAGATAAAATCGCTGAAA
AGAGCATAAAAACATCAAAATCAGATGATCTAACTCTCAATCTTGTAAAAGAGGGAAAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (54-269)

3D structure

• AlphaFold DB: A0A1Q2SMY6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.882	95.165	0.532
pilU	Acinetobacter baylyi ADP1	55.402	91.858	0.509
pilU	Vibrio cholerae strain A1552	49.857	88.804	0.443
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.319	87.786	0.372