Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MN093_RS03335 Genome accession   NZ_CP093291
Coordinates   635132..636175 (-) Length   347 a.a.
NCBI ID   WP_113734225.1    Uniprot ID   -
Organism   Bacillus mycoides strain DSM 2048     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 630132..641175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN093_RS03290 (MN093_03290) - 630530..630730 (-) 201 WP_002015009.1 YqzE family protein -
  MN093_RS03295 (MN093_03295) - 630769..631266 (-) 498 WP_002129082.1 shikimate kinase -
  MN093_RS03300 (MN093_03300) - 631418..632068 (-) 651 WP_113734229.1 2OG-Fe(II) oxygenase -
  MN093_RS03305 (MN093_03305) comGG 632243..632614 (-) 372 WP_131246343.1 competence type IV pilus minor pilin ComGG -
  MN093_RS03310 (MN093_03310) comGF 632611..633072 (-) 462 WP_044442604.1 competence type IV pilus minor pilin ComGF -
  MN093_RS03315 (MN093_03315) - 633056..633352 (-) 297 WP_016100615.1 hypothetical protein -
  MN093_RS03320 (MN093_03320) comGD 633345..633800 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  MN093_RS03325 (MN093_03325) comGC 633797..634096 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  MN093_RS03330 (MN093_03330) comGB 634108..635145 (-) 1038 WP_131246342.1 competence type IV pilus assembly protein ComGB -
  MN093_RS03335 (MN093_03335) comGA 635132..636175 (-) 1044 WP_113734225.1 competence type IV pilus ATPase ComGA Machinery gene
  MN093_RS03340 (MN093_03340) - 636381..637076 (+) 696 WP_002014995.1 helix-turn-helix transcriptional regulator -
  MN093_RS03345 (MN093_03345) - 637356..637598 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  MN093_RS03350 (MN093_03350) - 637706..639100 (+) 1395 WP_002138338.1 L-cystine transporter -
  MN093_RS03355 (MN093_03355) - 639181..639573 (-) 393 WP_060749607.1 hypothetical protein -
  MN093_RS03360 (MN093_03360) - 639661..639876 (-) 216 WP_002015115.1 DUF3912 family protein -
  MN093_RS03365 (MN093_03365) - 640129..640440 (+) 312 WP_002165070.1 hypothetical protein -
  MN093_RS03370 (MN093_03370) - 640476..640964 (-) 489 WP_241875775.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.78 Da        Isoelectric Point: 9.4590

>NTDB_id=663105 MN093_RS03335 WP_113734225.1 635132..636175(-) (comGA) [Bacillus mycoides strain DSM 2048]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGKEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=663105 MN093_RS03335 WP_113734225.1 635132..636175(-) (comGA) [Bacillus mycoides strain DSM 2048]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGAAAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTAGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363