Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKS87_RS02030 Genome accession   NZ_CP092631
Coordinates   373971..374420 (-) Length   149 a.a.
NCBI ID   WP_017696127.1    Uniprot ID   -
Organism   Bacillus subtilis strain YB-15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 368971..379420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKS87_RS01995 (MKS87_01995) yckA 369124..369804 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  MKS87_RS02000 (MKS87_02000) yckB 369814..370677 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  MKS87_RS02005 (MKS87_02005) - 370839..371036 (+) 198 WP_119122841.1 hypothetical protein -
  MKS87_RS02010 (MKS87_02010) yckC 371055..371510 (+) 456 WP_038428340.1 RDD family protein -
  MKS87_RS02015 (MKS87_02015) yckD 371592..371924 (+) 333 WP_015252849.1 YckD family protein -
  MKS87_RS02020 (MKS87_02020) bglC 372076..373509 (+) 1434 WP_038428341.1 6-phospho-beta-glucosidase -
  MKS87_RS02025 (MKS87_02025) nin/comJ 373546..373944 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  MKS87_RS02030 (MKS87_02030) nucA/comI 373971..374420 (-) 450 WP_017696127.1 DNA-entry nuclease Machinery gene
  MKS87_RS02035 (MKS87_02035) tlpC 374588..376309 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  MKS87_RS02040 (MKS87_02040) hxlB 376420..376977 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  MKS87_RS02045 (MKS87_02045) hxlA 376983..377615 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  MKS87_RS02050 (MKS87_02050) hxlR 377848..378210 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16412.34 Da        Isoelectric Point: 4.5282

>NTDB_id=659323 MKS87_RS02030 WP_017696127.1 373971..374420(-) (nucA/comI) [Bacillus subtilis strain YB-15]
MNITTDIIKTILLVIVIIAAAAAGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=659323 MKS87_RS02030 WP_017696127.1 373971..374420(-) (nucA/comI) [Bacillus subtilis strain YB-15]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGCCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGAT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98