Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   MBG98_RS09770 Genome accession   NZ_CP092038
Coordinates   2166880..2167293 (-) Length   137 a.a.
NCBI ID   WP_015444381.1    Uniprot ID   -
Organism   Legionella pneumophila strain 11052018-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2161880..2172293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MBG98_RS09760 (MBG98_09760) letS 2162810..2165542 (+) 2733 WP_010947629.1 two-component system sensor histidine kinase LetS Regulator
  MBG98_RS09765 (MBG98_09765) - 2165639..2166883 (+) 1245 WP_010947630.1 6-phosphofructokinase -
  MBG98_RS09770 (MBG98_09770) pilA2 2166880..2167293 (-) 414 WP_015444381.1 pilin Machinery gene
  MBG98_RS09775 (MBG98_09775) pilA2 2167490..2167900 (-) 411 WP_016356941.1 pilin Machinery gene
  MBG98_RS09780 (MBG98_09780) - 2168203..2168520 (+) 318 WP_010947633.1 BolA family protein -
  MBG98_RS09785 (MBG98_09785) - 2168595..2170016 (+) 1422 WP_010947634.1 APC family permease -
  MBG98_RS09790 (MBG98_09790) - 2170147..2171577 (-) 1431 WP_010947635.1 cellulase family glycosylhydrolase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14505.91 Da        Isoelectric Point: 6.2016

>NTDB_id=655301 MBG98_RS09770 WP_015444381.1 2166880..2167293(-) (pilA2) [Legionella pneumophila strain 11052018-5]
MRMKGFTLIELMIVAAILGILVAIAIPAYQDYTIRARVVEGLEMASAAKLAVVETSLIDNTLPANQADTGYTSPAPTINV
ASIAIGNQGIITITYTANAGNGTITMVPTIDATNRILTWDCTGGTLISKYRPAYCRP

Nucleotide


Download         Length: 414 bp        

>NTDB_id=655301 MBG98_RS09770 WP_015444381.1 2166880..2167293(-) (pilA2) [Legionella pneumophila strain 11052018-5]
ATGAGAATGAAAGGATTTACTTTAATCGAGCTGATGATCGTTGCCGCTATCCTTGGGATTCTGGTTGCCATCGCAATTCC
AGCCTATCAGGACTATACCATCAGAGCACGGGTGGTCGAAGGATTGGAAATGGCTTCGGCCGCTAAATTAGCTGTTGTTG
AAACGAGTTTAATTGATAACACTTTGCCAGCAAATCAGGCAGATACGGGCTATACCAGTCCTGCCCCAACAATTAATGTA
GCCTCTATAGCCATTGGTAATCAAGGCATTATTACCATTACCTACACTGCAAACGCAGGTAATGGTACCATTACAATGGT
TCCCACAATAGATGCCACTAACAGAATACTCACTTGGGATTGCACTGGTGGTACCCTGATTAGTAAATATCGTCCAGCCT
ACTGCCGCCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

72.993

100

0.73
  pilA2 Legionella pneumophila strain ERS1305867

72.263

100

0.723
  pilA Ralstonia pseudosolanacearum GMI1000

45.732

100

0.547
  comP Acinetobacter baylyi ADP1

45.89

100

0.489
  pilA/pilA1 Eikenella corrodens VA1

36.601

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.143

100

0.38
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.41

100

0.38
  pilA Vibrio cholerae C6706

34.899

100

0.38
  pilA Vibrio cholerae strain A1552

34.899

100

0.38
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.899

100

0.38