Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   L3D22_RS05670 Genome accession   NZ_CP091317
Coordinates   1269687..1270745 (+) Length   352 a.a.
NCBI ID   WP_115844053.1    Uniprot ID   A0A3D8V9N5
Organism   Lysobacter soli strain RCu6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1264687..1275745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3D22_RS05655 (L3D22_05650) - 1265405..1266016 (+) 612 WP_253337588.1 hypothetical protein -
  L3D22_RS05660 (L3D22_05655) - 1266038..1266691 (-) 654 WP_253337589.1 hypothetical protein -
  L3D22_RS05665 (L3D22_05660) - 1266913..1269405 (-) 2493 WP_115844055.1 penicillin-binding protein 1A -
  L3D22_RS05670 (L3D22_05665) pilM 1269687..1270745 (+) 1059 WP_115844053.1 pilus assembly protein PilM Machinery gene
  L3D22_RS05675 (L3D22_05670) - 1270745..1271530 (+) 786 WP_192631585.1 PilN domain-containing protein -
  L3D22_RS05680 (L3D22_05675) pilO 1271527..1272198 (+) 672 WP_192631584.1 type 4a pilus biogenesis protein PilO -
  L3D22_RS05685 (L3D22_05680) - 1272200..1272751 (+) 552 WP_192631583.1 pilus assembly protein PilP -
  L3D22_RS05690 (L3D22_05685) - 1272770..1274698 (+) 1929 WP_192631582.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37536.92 Da        Isoelectric Point: 4.4951

>NTDB_id=649121 L3D22_RS05670 WP_115844053.1 1269687..1270745(+) (pilM) [Lysobacter soli strain RCu6]
MGIVTKSQPAVVGVDISSTAVKLLQLSKAGNRYRVEHYAVEPLPPNAVVEKSIVEVEAVGEAIRRAVARSGTRAKHAAAA
VAGSSVITKVIPMPGDLDEDDLESQVELEAVNYVPYPIEEVNHDFEVLGPMPGSPDMLQVLLAASRSENVEVRASALEIG
GLTPKVMDVEAFALENAFALVADQLGGPRDGLIALVDSGATMTTLNVLRNGRSLYAREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEAEVLEPFKEAMVQQISRLLQFFYAGSEFNRVDQIVLAGGCASIAGIAEMVEEQLGVPTTIANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=649121 L3D22_RS05670 WP_115844053.1 1269687..1270745(+) (pilM) [Lysobacter soli strain RCu6]
GTGGGGATCGTCACAAAAAGCCAGCCGGCGGTCGTCGGCGTGGATATCAGTTCGACTGCCGTAAAGCTGTTGCAGCTCTC
CAAGGCCGGCAACCGCTATCGCGTCGAGCATTACGCGGTCGAGCCGCTGCCGCCGAACGCCGTCGTCGAGAAGAGCATCG
TCGAGGTCGAGGCCGTCGGCGAGGCGATCCGTCGCGCCGTCGCGCGTTCGGGCACTCGCGCCAAGCACGCTGCCGCCGCG
GTCGCGGGTTCGTCGGTCATCACCAAGGTCATCCCGATGCCGGGCGACCTGGATGAGGACGACCTCGAGTCGCAGGTCGA
GCTGGAGGCGGTGAACTACGTCCCGTATCCGATCGAAGAGGTCAACCACGATTTCGAAGTGCTGGGCCCGATGCCCGGCA
GCCCGGACATGCTGCAGGTTCTGCTTGCGGCGTCGCGTTCGGAGAACGTCGAAGTGCGCGCCTCGGCGCTGGAGATCGGC
GGCCTCACCCCGAAGGTGATGGACGTGGAGGCCTTCGCGCTCGAGAACGCCTTCGCGCTCGTCGCCGACCAGCTCGGCGG
TCCGCGCGACGGCCTCATCGCACTGGTCGATTCCGGCGCGACGATGACCACGCTCAACGTCCTGCGCAATGGCCGCAGCC
TGTATGCGCGCGAGCAGGTCTTCGGTGGCAAGCAGCTCACCGACGAAGTGATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGTGGTCTGCCGGAGAGCTATGAAGCCGAAGTGCTGGAGCCCTTCAAGGAGGCGATGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAATTCAACCGCGTCGACCAGATCGTCCTGGCCGGCG
GCTGCGCGTCCATCGCCGGCATCGCCGAGATGGTCGAGGAACAGCTCGGCGTGCCGACCACCATCGCCAACCCGCTGGCG
CACATGACGCTCGGCCCGCGCGTGCAGGCGCATGCGCTGGCGCAGGACGCCCCGGCCCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3D8V9N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.716

99.148

0.503
  comM Acinetobacter nosocomialis M2

50.43

99.148

0.5
  comM Acinetobacter baylyi ADP1

49.28

98.58

0.486
  pilM Legionella pneumophila strain ERS1305867

45.058

97.727

0.44