Detailed information NTG0644228

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L0026_RS02100
• Genome accession: NZ_CP090649
• Coordinates: 448614..449762 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA1609
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 443614..454762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L0026_RS02075 (L0026_02075)	-	444765..445061 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
L0026_RS02080 (L0026_02080)	-	445058..445651 (-)	594	WP_003084544.1	YggT family protein	-
L0026_RS02085 (L0026_02085)	proC	445662..446483 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
L0026_RS02090 (L0026_02090)	-	446495..447187 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
L0026_RS02095 (L0026_02095)	pilT	447402..448436 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
L0026_RS02100 (L0026_02100)	pilU	448614..449762 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
L0026_RS02105 (L0026_02105)	czcD	449770..450669 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
L0026_RS02110 (L0026_02110)	-	450823..451227 (+)	405	WP_003084560.1	NINE protein	-
L0026_RS02115 (L0026_02115)	-	451470..452843 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
L0026_RS02120 (L0026_02120)	-	452840..454024 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=644228 L0026_RS02100 WP_003100959.1 448614..449762(+) (pilU) [Pseudomonas aeruginosa strain PA1609]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=644228 L0026_RS02100 WP_003100959.1 448614..449762(+) (pilU) [Pseudomonas aeruginosa strain PA1609]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382