Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U712_RS12300 Genome accession   NC_022898
Coordinates   2470241..2470651 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis PY79     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2465241..2475651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U712_RS12270 (U712_12580) - 2465328..2465468 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  U712_RS12275 (U712_12585) yqeF 2465774..2466505 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  U712_RS12280 (U712_12590) cwlH 2466757..2467509 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  U712_RS12285 (U712_12595) yqeD 2467696..2468322 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  U712_RS12290 (U712_12600) gnd 2468341..2469234 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  U712_RS12295 (U712_12605) yqeB 2469486..2470208 (+) 723 WP_010886572.1 hypothetical protein -
  U712_RS12300 (U712_12610) nucA/comI 2470241..2470651 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  U712_RS12305 - 2470847..2471194 (+) 348 Protein_2460 sigma-70 family RNA polymerase sigma factor -
  U712_RS12310 (U712_12615) spoIVCA 2471225..2472685 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  U712_RS21590 - 2472643..2472821 (-) 179 Protein_2462 hypothetical protein -
  U712_RS12315 (U712_12620) arsC 2473176..2473595 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  U712_RS12320 (U712_12625) acr3 2473607..2474647 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  U712_RS12325 (U712_12630) arsK 2474670..2475110 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  U712_RS12330 (U712_12635) arsR 2475171..2475488 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=63762 U712_RS12300 WP_009967785.1 2470241..2470651(-) (nucA/comI) [Bacillus subtilis PY79]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=63762 U712_RS12300 WP_009967785.1 2470241..2470651(-) (nucA/comI) [Bacillus subtilis PY79]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment