Detailed information NTG0063538

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PA1R_RS12555
• Genome accession: NC_022806
• Coordinates: 2719234..2720106 (+)
• Length: 290 a.a.
• NCBI ID: WP_009875878.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa PA1R
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2714234..2725106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA1R_RS12530 (PA1R_gp2425)	nadC	2714575..2715423 (+)	849	WP_003161760.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PA1R_RS12540 (PA1R_gp2427)	pilA	2715622..2716074 (-)	453	WP_023518088.1	pilin	Machinery gene
PA1R_RS12545 (PA1R_gp2428)	pilB	2716306..2718006 (+)	1701	WP_021264132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PA1R_RS12550 (PA1R_gp2429)	pilC	2718010..2719230 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
PA1R_RS12555 (PA1R_gp2430)	pilD	2719234..2720106 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PA1R_RS12560 (PA1R_gp2431)	coaE	2720103..2720714 (+)	612	WP_023518089.1	dephospho-CoA kinase	-
PA1R_RS12565	yacG	2720711..2720911 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PA1R_RS12570 (PA1R_gp2432)	-	2720948..2721157 (-)	210	WP_003094660.1	hypothetical protein	-
PA1R_RS12575 (PA1R_gp2433)	-	2721263..2721952 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
PA1R_RS12580 (PA1R_gp2434)	-	2721949..2722419 (-)	471	WP_003103866.1	hypothetical protein	-
PA1R_RS12585 (PA1R_gp2435)	-	2722416..2722841 (-)	426	WP_016562341.1	GNAT family N-acetyltransferase	-
PA1R_RS12590 (PA1R_gp2436)	-	2722974..2723603 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
PA1R_RS12595 (PA1R_gp2437)	-	2723600..2724049 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
PA1R_RS12600	-	2724075..2724245 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31811.84 Da
• Isoelectric Point: 7.7068

>NTDB_id=63538 PA1R_RS12555 WP_009875878.1 2719234..2720106(+) (pilD) [Pseudomonas aeruginosa PA1R]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLASYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=63538 PA1R_RS12555 WP_009875878.1 2719234..2720106(+) (pilD) [Pseudomonas aeruginosa PA1R]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGCCAGCTACCTGGCGCTGGGCGGCAAGTGTTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATACTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469