Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LSG23_RS08585 Genome accession   NZ_CP088973
Coordinates   1790775..1791209 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain CMML20-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1785775..1796209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG23_RS08580 (LSG23_08560) - 1789275..1790486 (-) 1212 WP_077723156.1 cytochrome P450 -
  LSG23_RS08585 (LSG23_08565) nucA/comI 1790775..1791209 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LSG23_RS08590 (LSG23_08570) - 1791269..1792024 (+) 756 WP_077722226.1 YoaK family protein -
  LSG23_RS08595 (LSG23_08575) - 1792058..1792420 (-) 363 WP_077722227.1 hypothetical protein -
  LSG23_RS08600 (LSG23_08580) - 1792613..1793941 (-) 1329 WP_043867149.1 S8 family peptidase -
  LSG23_RS08605 (LSG23_08585) - 1794120..1794353 (+) 234 WP_015239897.1 hypothetical protein -
  LSG23_RS08610 (LSG23_08590) - 1794609..1795316 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  LSG23_RS08615 (LSG23_08595) - 1795377..1795829 (+) 453 WP_070081858.1 OsmC family protein -
  LSG23_RS08620 (LSG23_08600) - 1795843..1796196 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=634300 LSG23_RS08585 WP_007611576.1 1790775..1791209(+) (nucA/comI) [Bacillus velezensis strain CMML20-16]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=634300 LSG23_RS08585 WP_007611576.1 1790775..1791209(+) (nucA/comI) [Bacillus velezensis strain CMML20-16]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCAGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486