Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LPA45_RS01730 Genome accession   NZ_CP088083
Coordinates   361850..363328 (-) Length   492 a.a.
NCBI ID   WP_116317023.1    Uniprot ID   -
Organism   Cupriavidus sp. Agwp_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356850..368328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA45_RS01700 (LPA45_01695) - 357279..357947 (-) 669 WP_367394836.1 LON peptidase substrate-binding domain-containing protein -
  LPA45_RS01705 (LPA45_01700) rapZ 358057..358941 (-) 885 WP_116317020.1 RNase adapter RapZ -
  LPA45_RS01710 (LPA45_01705) - 359063..359395 (-) 333 WP_116317021.1 PsiF family protein -
  LPA45_RS01715 (LPA45_01710) hprK 359573..360544 (-) 972 WP_063236682.1 HPr(Ser) kinase/phosphatase -
  LPA45_RS01720 (LPA45_01715) ptsN 360625..361080 (-) 456 WP_116317022.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LPA45_RS01725 (LPA45_01720) hpf 361451..361801 (-) 351 WP_029046778.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LPA45_RS01730 (LPA45_01725) rpoN/rpoN1 361850..363328 (-) 1479 WP_116317023.1 RNA polymerase factor sigma-54 Machinery gene
  LPA45_RS01735 (LPA45_01730) lptB 363484..364284 (-) 801 WP_116317024.1 LPS export ABC transporter ATP-binding protein -
  LPA45_RS01740 (LPA45_01735) lptA 364338..364985 (-) 648 WP_116317025.1 lipopolysaccharide transport periplasmic protein LptA -
  LPA45_RS01745 (LPA45_01740) lptC 365124..365753 (-) 630 WP_116317026.1 LPS export ABC transporter periplasmic protein LptC -
  LPA45_RS01750 (LPA45_01745) - 365776..366759 (-) 984 WP_116317811.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54738.09 Da        Isoelectric Point: 4.9169

>NTDB_id=632613 LPA45_RS01730 WP_116317023.1 361850..363328(-) (rpoN/rpoN1) [Cupriavidus sp. Agwp_2]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARADGAGDDDSYGDSNSGDDYGGDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISVRDKGLAIF
LIESLDDDGYLSASLEEICAELPEELEFEIDEVQAMLTLLQSFDPPGVGARNAAECLGLQLRRLKHPQRELALNIVHNHL
ELLAVRDYTRLKKALQVDEPALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGGGWIAQLNPDVMPRLRINDMYA
QILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=632613 LPA45_RS01730 WP_116317023.1 361850..363328(-) (rpoN/rpoN1) [Cupriavidus sp. Agwp_2]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCACCTGGCACTGACGCCGCAACTGCAGCAGTCGATCAGGCTGTTGCA
GCTGTCGACGCTGGAACTGCAGCAGGAAGTCGAACAGGCGCTCACGGAAAACCCGCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCTGCCGATGGTTCGGTCAACTTGCAGAGCGCGCCGGCCCCGGCACCAGCCGAACCGCAA
GGCAACGGCGAGGCGCGCGCCGATGGCGCGGGCGACGACGACAGCTACGGCGACAGCAACAGCGGCGATGACTATGGCGG
CGACTGGAGCCTGGACGACTTCGCGCGCCGCCCGCAAGGCGACGAAGACGAGAAGACGCCGATGCAGCTGCGCGAGGCCG
AGCCCACGCTGCGCGAATACCTGATGGAACAGCTGACGCCGCTGAAGATTTCGGTGCGCGACAAGGGCCTTGCCATCTTC
CTGATCGAATCACTCGACGACGACGGCTACCTCAGCGCCTCACTCGAAGAAATCTGCGCAGAACTGCCCGAAGAACTCGA
GTTCGAGATCGACGAAGTGCAGGCCATGCTGACGCTGCTGCAGAGCTTCGACCCGCCTGGCGTGGGCGCGCGCAATGCCG
CCGAGTGCCTCGGCCTGCAGTTGCGCCGGCTGAAGCACCCGCAGCGCGAGCTGGCGCTGAACATCGTGCATAACCACCTG
GAACTGCTGGCAGTGCGCGACTACACGCGACTGAAGAAAGCGCTGCAGGTAGACGAGCCAGCACTCAAGGCCGCGCACGA
ATTGATCCGCTCGCTGGCACCTTACCCCGGCCACGCGTATAGCCGCCCCGAAGCCGACTTCGTCGTGCCCGATGTGTTCG
TGCGCAAGAGCGGCGGCGGCTGGATCGCGCAACTCAATCCGGATGTGATGCCGAGGCTGCGCATCAACGACATGTACGCG
CAGATCCTGCGCGGCGCGAAGGGAGAGTCAGGTACCGCTGGCCTGCAGCAGAAGCTGCAGGAGGCACGCTGGCTGATCAA
GAATATCCAGCAAAGGTTCGACACGATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCACGCACG
GCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATCTCCCGGGTG
ACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGGAGCCACGTGTCCACCGAAAC
CGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCCTTT
CCGACAGTCGCATCGCCGAACTGCTGGGTGAGCAAGGCTTCGTTGTCGCACGCCGCACCGTTGCCAAATATCGCGAAGCC
CTGAAGATCCCCGCAGTGAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

80.24

100

0.817