Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   N5855_RS14510 Genome accession   NZ_CP104629
Coordinates   3021674..3023167 (-) Length   497 a.a.
NCBI ID   WP_000249348.1    Uniprot ID   B7LGA7
Organism   Escherichia coli strain AHM21C2872I     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3016674..3028167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5855_RS14480 (N5855_14440) gspK 3017468..3018445 (-) 978 WP_021557197.1 type II secretion system minor pseudopilin GspK -
  N5855_RS14485 (N5855_14445) gspJ 3018442..3019047 (-) 606 WP_021557198.1 type II secretion system minor pseudopilin GspJ -
  N5855_RS14490 (N5855_14450) gspI 3019044..3019415 (-) 372 WP_021557199.1 type II secretion system minor pseudopilin GspI -
  N5855_RS14495 (N5855_14455) gspH 3019412..3019975 (-) 564 WP_001613816.1 type II secretion system minor pseudopilin GspH -
  N5855_RS14500 (N5855_14460) gspG 3019979..3020434 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  N5855_RS14505 (N5855_14465) gspF 3020451..3021674 (-) 1224 WP_021557200.1 type II secretion system inner membrane protein GspF -
  N5855_RS14510 (N5855_14470) pilB/pilB1 3021674..3023167 (-) 1494 WP_000249348.1 type II secretion system ATPase GspE Machinery gene
  N5855_RS14515 (N5855_14475) gspD 3023167..3025227 (-) 2061 WP_137535887.1 type II secretion system secretin GspD -
  N5855_RS14520 (N5855_14480) gspC 3025257..3026216 (-) 960 WP_024227187.1 type II secretion system protein GspC -
  N5855_RS14525 (N5855_14485) gspS2 3026234..3026644 (-) 411 WP_021557202.1 type II secretion system pilot lipoprotein GspS-beta -
  N5855_RS14530 (N5855_14490) pilD 3026710..3027519 (-) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.36 Da        Isoelectric Point: 6.0390

>NTDB_id=631895 N5855_RS14510 WP_000249348.1 3021674..3023167(-) (pilB/pilB1) [Escherichia coli strain AHM21C2872I]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=631895 N5855_RS14510 WP_000249348.1 3021674..3023167(-) (pilB/pilB1) [Escherichia coli strain AHM21C2872I]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTAATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTAACGGGGCCGACGGGTTCCGGAAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAATATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGAATTGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCGCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCATAAAGGTTTCCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362