Detailed information NTG0627380

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY57_RS11885
• Genome accession: NZ_CP087177
• Coordinates: 2746315..2747424 (+)
• Length: 369 a.a.
• NCBI ID: WP_258641307.1
• Uniprot ID: -
• Organism: Pseudomonas moraviensis strain B21-039
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2741315..2752424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY57_RS11870 (LOY57_11840)	-	2742301..2743224 (+)	924	WP_258641305.1	sugar kinase	-
LOY57_RS11875 (LOY57_11845)	-	2743340..2743615 (-)	276	WP_007912322.1	peptidylprolyl isomerase	-
LOY57_RS11880 (LOY57_11850)	-	2743668..2746205 (-)	2538	WP_258641306.1	PAS domain-containing sensor histidine kinase	-
LOY57_RS11885 (LOY57_11855)	pilU	2746315..2747424 (+)	1110	WP_258641307.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY57_RS11890 (LOY57_11860)	-	2747552..2747938 (+)	387	WP_258641308.1	hypothetical protein	-
LOY57_RS11895 (LOY57_11865)	-	2748029..2751193 (+)	3165	WP_258641309.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41047.89 Da
• Isoelectric Point: 6.8945

>NTDB_id=627380 LOY57_RS11885 WP_258641307.1 2746315..2747424(+) (pilU) [Pseudomonas moraviensis strain B21-039]
MEIDALLSTLSQKNGSDLFLSTGAPPSARVDGVLTALNDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEERREQLLQTLGNNLKACVSQRLVRTVDGQRRAAVEVMLGNPTIEDHIRRNELGELKGVMEKSIDLGMQTFDGAL
YNLVLEGAITMEEAFKNADSINNIRLRLKLSGESIDAQGRHSGGWSLLE

Nucleotide

• Length: 1110 bp

>NTDB_id=627380 LOY57_RS11885 WP_258641307.1 2746315..2747424(+) (pilU) [Pseudomonas moraviensis strain B21-039]
ATGGAAATCGATGCACTGTTATCAACATTGTCGCAAAAAAACGGTTCGGATTTGTTCCTCTCCACCGGCGCACCGCCCAG
CGCGCGCGTTGACGGTGTGTTGACGGCATTGAACGATCAGCCATTCAAGCCCGGCGAAACCGCGGCAATCGCCACCTCCC
TGATGGACGCTGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTCTCGATCGTCATCCGCAACGTCAAACTCGACATTCCGCGATTCGA
AGACTTGAAGCTGCCGCCAGTGTTGCTCGAGACGGTGATGCTCAAACGCGGCCTGATTCTCTTCGTCGGCGCCACCAGTT
CAGGCAAGTCAACCTCGCTGGCCGCCCTGATCGACCACCGCAACCGCCACAGTGCCGGGCACATCGTGACCATCGAGGAC
CCGATCGAATATATCCATCGGCACAAGCGTTCGATCATCAACCAGCGTGAAGTCGGCGTCGACACGCGCAGCTTTCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTCGTATTGATCGGCGAAATCCGTGATCGCGAAACCATGGAACATG
CATTGTCATTCGCCGATACCGGGCATCTGGTGCTGTCGACGTTGCACGCGACCAATGCCAATCAGGCACTCGATCGAATC
ATCAATATGTTTCCCGAGGAGCGGCGCGAGCAGCTTTTGCAAACCCTGGGCAACAACCTGAAGGCTTGTGTATCGCAAAG
ACTTGTACGCACGGTTGACGGGCAGAGAAGAGCAGCGGTGGAAGTCATGCTGGGGAATCCGACTATTGAAGATCACATTC
GCAGAAATGAGTTGGGTGAGTTGAAAGGCGTAATGGAAAAATCCATCGATTTGGGCATGCAGACCTTTGATGGTGCTCTT
TACAACCTGGTTCTGGAGGGGGCGATCACTATGGAAGAAGCATTCAAGAATGCAGACTCCATAAATAACATCAGGCTGCG
ATTAAAACTTTCCGGCGAATCAATTGATGCGCAGGGGCGCCATTCTGGCGGCTGGAGTTTGTTGGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	96.206	0.545
pilU	Acinetobacter baylyi ADP1	51.685	96.477	0.499
pilU	Vibrio cholerae strain A1552	51.143	94.851	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.708	91.87	0.374
pilT	Pseudomonas aeruginosa PAK	39.535	93.225	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Acinetobacter baumannii strain A118	40.299	90.786	0.366
pilT	Acinetobacter baumannii D1279779	40.299	90.786	0.366
pilT	Acinetobacter nosocomialis M2	40.299	90.786	0.366
pilT	Neisseria gonorrhoeae MS11	39.823	91.87	0.366
pilT	Neisseria meningitidis 8013	39.589	92.412	0.366
pilT	Acinetobacter baylyi ADP1	39.412	92.141	0.363
pilT	Legionella pneumophila strain Lp02	39.645	91.599	0.363
pilT	Legionella pneumophila strain ERS1305867	39.645	91.599	0.363