Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIS78_RS19790 Genome accession   NZ_CP085442
Coordinates   3806181..3806555 (+) Length   124 a.a.
NCBI ID   WP_229754616.1    Uniprot ID   -
Organism   Priestia megaterium strain KF18     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3801181..3811555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS78_RS19770 (LIS78_19790) - 3801518..3802189 (-) 672 WP_195782177.1 response regulator transcription factor -
  LIS78_RS19775 (LIS78_19795) - 3802515..3803333 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  LIS78_RS19780 (LIS78_19800) - 3803369..3805318 (-) 1950 WP_252284245.1 FUSC family protein -
  LIS78_RS19785 (LIS78_19805) - 3805475..3805735 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  LIS78_RS19790 (LIS78_19810) nucA/comI 3806181..3806555 (+) 375 WP_229754616.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LIS78_RS19795 (LIS78_19815) comJ 3806591..3806998 (+) 408 WP_209150528.1 competence protein ComJ -
  LIS78_RS19800 (LIS78_19820) helD 3807030..3809348 (-) 2319 WP_252284246.1 RNA polymerase recycling motor HelD -
  LIS78_RS31475 - 3809592..3809717 (-) 126 WP_016765178.1 hypothetical protein -
  LIS78_RS19805 (LIS78_19825) - 3809858..3810376 (-) 519 WP_252284247.1 enoyl-CoA hydratase/isomerase family protein -
  LIS78_RS19810 (LIS78_19830) - 3810616..3810984 (+) 369 WP_195782172.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13675.93 Da        Isoelectric Point: 4.2601

>NTDB_id=618145 LIS78_RS19790 WP_229754616.1 3806181..3806555(+) (nucA/comI) [Priestia megaterium strain KF18]
MGHAAFLAPEKTEAEINYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=618145 LIS78_RS19790 WP_229754616.1 3806181..3806555(+) (nucA/comI) [Priestia megaterium strain KF18]
ATGGGACATGCGGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAATAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCAACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGGGGAGCCGGCTCGTGGGTTGG
AAATCAATTAGAACAGTACCCAGATGGTACGCGCGTACAATTTATCGTCCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645