Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NMT99_RS12970 Genome accession   NZ_CP101290
Coordinates   2512197..2512607 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain LjM2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2507197..2517607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS12940 - 2507309..2507449 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NMT99_RS12945 yqeF 2507755..2508486 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  NMT99_RS12950 cwlH 2508738..2509490 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NMT99_RS12955 yqeD 2509677..2510303 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  NMT99_RS12960 gnd 2510322..2511190 (-) 869 Protein_2507 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NMT99_RS12965 yqeB 2511442..2512164 (+) 723 WP_010886572.1 hypothetical protein -
  NMT99_RS12970 nucA/comI 2512197..2512607 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NMT99_RS12975 - 2512803..2513153 (+) 351 Protein_2510 sigma-70 family RNA polymerase sigma factor -
  NMT99_RS12980 spoIVCA 2513181..2514641 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  NMT99_RS12985 - 2514599..2514777 (-) 179 Protein_2512 hypothetical protein -
  NMT99_RS12990 arsC 2515132..2515551 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  NMT99_RS12995 acr3 2515563..2516603 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  NMT99_RS13000 arsK 2516626..2517066 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  NMT99_RS13005 arsR 2517114..2517431 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=610029 NMT99_RS12970 WP_009967785.1 2512197..2512607(-) (nucA/comI) [Bacillus subtilis strain LjM2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=610029 NMT99_RS12970 WP_009967785.1 2512197..2512607(-) (nucA/comI) [Bacillus subtilis strain LjM2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529