Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LAZ96_RS02885 Genome accession   NZ_CP083715
Coordinates   546731..547345 (-) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain DMB05     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541731..552345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ96_RS02855 (LAZ96_02855) rpsT 542034..542300 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  LAZ96_RS02860 (LAZ96_02860) holA 542317..543358 (-) 1042 Protein_567 DNA polymerase III subunit delta -
  LAZ96_RS02865 (LAZ96_02865) - 543398..543549 (-) 152 Protein_568 hypothetical protein -
  LAZ96_RS02870 (LAZ96_02870) - 543590..543724 (+) 135 WP_003152870.1 YqzM family protein -
  LAZ96_RS02875 (LAZ96_02875) comEC 543743..546094 (-) 2352 WP_017418179.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LAZ96_RS02880 (LAZ96_02880) - 546095..546664 (-) 570 WP_003152868.1 ComE operon protein 2 -
  LAZ96_RS02885 (LAZ96_02885) comEA 546731..547345 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  LAZ96_RS02890 (LAZ96_02890) comER 547404..548225 (+) 822 WP_014305449.1 late competence protein ComER -
  LAZ96_RS02895 (LAZ96_02895) - 548294..549031 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  LAZ96_RS02900 (LAZ96_02900) rsfS 549028..549384 (-) 357 WP_003152864.1 ribosome silencing factor -
  LAZ96_RS02905 (LAZ96_02905) yqeK 549381..549962 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LAZ96_RS02910 (LAZ96_02910) - 549952..550521 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  LAZ96_RS02915 (LAZ96_02915) yhbY 550532..550822 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  LAZ96_RS02920 (LAZ96_02920) aroE 550816..551658 (-) 843 WP_017418183.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=607388 LAZ96_RS02885 WP_013352942.1 546731..547345(-) (comEA) [Bacillus velezensis strain DMB05]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=607388 LAZ96_RS02885 WP_013352942.1 546731..547345(-) (comEA) [Bacillus velezensis strain DMB05]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363