Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LAG74_RS07345 Genome accession   NZ_CP083461
Coordinates   1586887..1588026 (-) Length   379 a.a.
NCBI ID   WP_005353124.1    Uniprot ID   A0A0T6QZZ8
Organism   Aeromonas veronii strain SW3814     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1581887..1593026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAG74_RS07325 (LAG74_07340) oppF 1582626..1583621 (+) 996 WP_005347159.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  LAG74_RS07330 (LAG74_07345) - 1583924..1584868 (+) 945 WP_005337664.1 TerC family protein -
  LAG74_RS07335 (LAG74_07350) alr 1584935..1586161 (-) 1227 WP_224969786.1 alanine racemase -
  LAG74_RS07340 (LAG74_07355) - 1586335..1586784 (+) 450 WP_224969787.1 isoprenylcysteine carboxylmethyltransferase family protein -
  LAG74_RS07345 (LAG74_07360) pilU 1586887..1588026 (-) 1140 WP_005353124.1 type IVa pilus ATPase TapW Machinery gene
  LAG74_RS07350 (LAG74_07365) - 1588085..1588441 (-) 357 WP_005337656.1 DMT family protein -
  LAG74_RS07355 (LAG74_07370) - 1588554..1589285 (+) 732 WP_224969788.1 transporter substrate-binding domain-containing protein -
  LAG74_RS07360 (LAG74_07375) btuC 1589383..1590390 (+) 1008 WP_005353116.1 vitamin B12 ABC transporter permease BtuC -
  LAG74_RS07365 (LAG74_07380) - 1590456..1591253 (+) 798 WP_224970182.1 ATP-binding cassette domain-containing protein -
  LAG74_RS07370 (LAG74_07385) - 1591370..1592431 (-) 1062 WP_224969789.1 3-deoxy-7-phosphoheptulonate synthase -
  LAG74_RS07375 (LAG74_07390) - 1592576..1592956 (+) 381 WP_005337641.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42253.34 Da        Isoelectric Point: 6.6011

>NTDB_id=606012 LAG74_RS07345 WP_005353124.1 1586887..1588026(-) (pilU) [Aeromonas veronii strain SW3814]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=606012 LAG74_RS07345 WP_005353124.1 1586887..1588026(-) (pilU) [Aeromonas veronii strain SW3814]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTTGTGGCCCGCAACATCAAGACCGAAATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGTAAATCCACTTCGCTGGCGGCGCTTATCGACCATCGCAACCGCAACAGCGGTGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGTAGCTTCCATGC
TGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGTCGCCGGGCCGCAGTGGAGATCATGCTTGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGTGGTCTCAAAGAGGTGATGGAGAAATCCAAAGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTTGACCTGGTAGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGACAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6QZZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367