Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | ABWED_RS11485 | Genome accession | NZ_CP082143 |
| Coordinates | 2366399..2367520 (+) | Length | 373 a.a. |
| NCBI ID | WP_004279277.1 | Uniprot ID | N9QN87 |
| Organism | Acinetobacter lwoffii strain ED23-35 | ||
| Function | mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 2334131..2410171 | 2366399..2367520 | within | 0 |
Gene organization within MGE regions
Location: 2334131..2410171
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ABWED_RS11350 (ABWED_2364) | - | 2334206..2335639 (+) | 1434 | WP_085064208.1 | trehalose-6-phosphate synthase | - |
| ABWED_RS11355 (ABWED_2365) | - | 2335665..2336909 (-) | 1245 | WP_005251197.1 | MFS transporter | - |
| ABWED_RS11360 (ABWED_2366) | - | 2337115..2337828 (-) | 714 | WP_005251195.1 | glycosyltransferase family 2 protein | - |
| ABWED_RS11365 (ABWED_2367) | - | 2337825..2338439 (-) | 615 | WP_005251192.1 | class I SAM-dependent methyltransferase | - |
| ABWED_RS11370 (ABWED_2368) | - | 2338429..2339214 (-) | 786 | WP_005251190.1 | PIG-L deacetylase family protein | - |
| ABWED_RS11375 (ABWED_2369) | - | 2339202..2340188 (-) | 987 | WP_005251188.1 | hypothetical protein | - |
| ABWED_RS11380 (ABWED_2370) | - | 2340237..2340446 (-) | 210 | WP_004646201.1 | hypothetical protein | - |
| ABWED_RS11385 (ABWED_2371) | katE | 2340518..2342641 (-) | 2124 | WP_005251185.1 | catalase HPII | - |
| ABWED_RS11390 (ABWED_2372) | - | 2343402..2343902 (+) | 501 | WP_004646199.1 | Dps family protein | - |
| ABWED_RS11395 (ABWED_2373) | - | 2344232..2344948 (-) | 717 | WP_005251184.1 | ABC transporter ATP-binding protein | - |
| ABWED_RS11400 (ABWED_2374) | - | 2344941..2346164 (-) | 1224 | WP_085064209.1 | ABC transporter permease | - |
| ABWED_RS11405 (ABWED_2375) | - | 2346161..2347384 (-) | 1224 | WP_005251180.1 | efflux RND transporter periplasmic adaptor subunit | - |
| ABWED_RS11410 (ABWED_2376) | - | 2347714..2349237 (-) | 1524 | WP_085064210.1 | M81 family metallopeptidase | - |
| ABWED_RS11415 (ABWED_2377) | - | 2349269..2350597 (-) | 1329 | WP_005251177.1 | MFS transporter | - |
| ABWED_RS11420 (ABWED_2378) | - | 2350769..2351653 (+) | 885 | WP_374955995.1 | LysR family transcriptional regulator | - |
| ABWED_RS11430 (ABWED_2379) | glpK | 2352209..2353693 (+) | 1485 | WP_374955996.1 | glycerol kinase GlpK | - |
| ABWED_RS11435 (ABWED_2380) | - | 2353773..2354270 (-) | 498 | WP_004646146.1 | thioesterase family protein | - |
| ABWED_RS11440 (ABWED_2381) | hemB | 2354376..2355389 (+) | 1014 | WP_005267344.1 | porphobilinogen synthase | - |
| ABWED_RS11445 (ABWED_2382) | thiO | 2355405..2356529 (+) | 1125 | WP_005267345.1 | glycine oxidase ThiO | - |
| ABWED_RS11450 (ABWED_2383) | - | 2356518..2357423 (-) | 906 | WP_374955997.1 | TIGR01777 family oxidoreductase | - |
| ABWED_RS11455 (ABWED_2384) | - | 2357579..2358037 (-) | 459 | WP_004730552.1 | ABZJ_00895 family protein | - |
| ABWED_RS11460 (ABWED_2386) | - | 2358507..2359760 (+) | 1254 | WP_374955998.1 | exonuclease SbcCD subunit D | - |
| ABWED_RS11465 (ABWED_2387) | - | 2359769..2363374 (+) | 3606 | WP_374955999.1 | AAA family ATPase | - |
| ABWED_RS11470 (ABWED_2388) | - | 2363377..2364486 (+) | 1110 | WP_374956000.1 | acyltransferase family protein | - |
| ABWED_RS11475 (ABWED_2389) | - | 2364518..2365207 (-) | 690 | WP_374956232.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| ABWED_RS11480 (ABWED_2390) | pilT | 2365331..2366368 (+) | 1038 | WP_004279279.1 | type IV pilus twitching motility protein PilT | Machinery gene |
| ABWED_RS11485 (ABWED_2391) | pilU | 2366399..2367520 (+) | 1122 | WP_004279277.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| ABWED_RS11490 (ABWED_2392) | fur | 2367588..2368025 (-) | 438 | WP_004279276.1 | ferric iron uptake transcriptional regulator | - |
| ABWED_RS11495 (ABWED_2393) | - | 2368138..2368536 (+) | 399 | WP_004279275.1 | outer membrane protein assembly factor BamE | - |
| ABWED_RS11500 (ABWED_2394) | - | 2368637..2368969 (-) | 333 | WP_005104788.1 | RnfH family protein | - |
| ABWED_RS11505 (ABWED_2395) | - | 2368969..2370048 (-) | 1080 | WP_374956001.1 | hypothetical protein | - |
| ABWED_RS11510 (ABWED_2396) | - | 2370192..2370647 (-) | 456 | WP_374956002.1 | bacteriohemerythrin | - |
| ABWED_RS11515 (ABWED_2397) | - | 2370923..2371771 (-) | 849 | WP_004646129.1 | class II glutamine amidotransferase | - |
| ABWED_RS11520 (ABWED_2398) | argB | 2371903..2372811 (-) | 909 | WP_005104791.1 | acetylglutamate kinase | - |
| ABWED_RS11525 (ABWED_2399) | - | 2372822..2374240 (-) | 1419 | WP_243475293.1 | phosphomannomutase/phosphoglucomutase | - |
| ABWED_RS11530 | - | 2374491..2374961 (-) | 471 | WP_004646477.1 | IS5/IS1182 family transposase | - |
| ABWED_RS11535 (ABWED_2401) | - | 2374909..2375115 (-) | 207 | WP_374956003.1 | hypothetical protein | - |
| ABWED_RS11540 (ABWED_2402) | - | 2375264..2376232 (+) | 969 | WP_081398964.1 | IS30 family transposase | - |
| ABWED_RS11545 (ABWED_2403) | dut | 2376635..2377087 (-) | 453 | WP_005247107.1 | dUTP diphosphatase | - |
| ABWED_RS11550 (ABWED_2404) | - | 2377135..2379174 (-) | 2040 | WP_005107705.1 | UvrD-helicase domain-containing protein | - |
| ABWED_RS11555 (ABWED_2405) | - | 2379311..2379964 (-) | 654 | WP_004730587.1 | OmpA family protein | - |
| ABWED_RS11560 (ABWED_2406) | - | 2380217..2381158 (+) | 942 | WP_374956233.1 | acyltransferase | - |
| ABWED_RS11565 (ABWED_2407) | - | 2381161..2382225 (+) | 1065 | WP_374956004.1 | preprotein translocase subunit SecA | - |
| ABWED_RS11570 (ABWED_2408) | minC | 2382385..2383098 (+) | 714 | WP_138742932.1 | septum site-determining protein MinC | - |
| ABWED_RS11575 (ABWED_2409) | minD | 2383165..2383977 (+) | 813 | WP_004646120.1 | septum site-determining protein MinD | - |
| ABWED_RS11580 (ABWED_2410) | minE | 2383980..2384252 (+) | 273 | WP_004279256.1 | cell division topological specificity factor MinE | - |
| ABWED_RS11585 (ABWED_2411) | - | 2384449..2384736 (+) | 288 | WP_004279254.1 | PA4642 family protein | - |
| ABWED_RS11590 (ABWED_2412) | - | 2384798..2387854 (-) | 3057 | WP_374956005.1 | DNA translocase FtsK 4TM domain-containing protein | - |
| ABWED_RS11595 (ABWED_2413) | trxB | 2388104..2389066 (+) | 963 | WP_253160813.1 | thioredoxin-disulfide reductase | - |
| ABWED_RS11600 (ABWED_2414) | aat | 2389159..2389890 (+) | 732 | WP_253160811.1 | leucyl/phenylalanyl-tRNA--protein transferase | - |
| ABWED_RS11605 (ABWED_2415) | - | 2389912..2390727 (+) | 816 | WP_071850221.1 | arginyltransferase | - |
| ABWED_RS11610 (ABWED_2416) | rimI | 2390743..2391201 (-) | 459 | WP_114541553.1 | ribosomal protein S18-alanine N-acetyltransferase | - |
| ABWED_RS11615 (ABWED_2417) | - | 2391307..2392188 (-) | 882 | WP_374956006.1 | metal-dependent hydrolase | - |
| ABWED_RS11620 (ABWED_2418) | tuf | 2392412..2393602 (-) | 1191 | WP_004646112.1 | elongation factor Tu | - |
| ABWED_RS11625 (ABWED_2419) | fusA | 2393695..2395830 (-) | 2136 | WP_180181093.1 | elongation factor G | - |
| ABWED_RS11630 (ABWED_2420) | rpsG | 2395959..2396429 (-) | 471 | WP_004279240.1 | 30S ribosomal protein S7 | - |
| ABWED_RS11635 (ABWED_2421) | rpsL | 2396610..2396984 (-) | 375 | WP_002050319.1 | 30S ribosomal protein S12 | - |
| ABWED_RS11640 (ABWED_2422) | - | 2397140..2398624 (-) | 1485 | WP_374956007.1 | EcsC family protein | - |
| ABWED_RS11645 (ABWED_2423) | - | 2398898..2400127 (+) | 1230 | WP_004730606.1 | beta-ketoacyl synthase N-terminal-like domain-containing protein | - |
| ABWED_RS11650 (ABWED_2424) | - | 2400151..2400603 (+) | 453 | WP_004730607.1 | CopD family protein | - |
| ABWED_RS11655 (ABWED_2425) | - | 2400663..2402090 (-) | 1428 | WP_374956008.1 | amino acid permease | - |
| ABWED_RS11660 (ABWED_2426) | - | 2402259..2402624 (-) | 366 | WP_114541406.1 | RidA family protein | - |
| ABWED_RS11665 (ABWED_2427) | alr | 2402673..2403785 (-) | 1113 | WP_168386742.1 | alanine racemase | - |
| ABWED_RS11670 (ABWED_2428) | - | 2403820..2405079 (-) | 1260 | WP_004646103.1 | D-amino acid dehydrogenase | - |
| ABWED_RS11675 (ABWED_2429) | - | 2405220..2405687 (+) | 468 | WP_004279232.1 | Lrp/AsnC ligand binding domain-containing protein | - |
| ABWED_RS11680 (ABWED_2430) | lysM | 2405775..2406269 (-) | 495 | WP_338840793.1 | peptidoglycan-binding protein LysM | - |
| ABWED_RS11685 (ABWED_2431) | acpP | 2406455..2406691 (-) | 237 | WP_004646101.1 | acyl carrier protein | - |
| ABWED_RS11690 (ABWED_2432) | fabG | 2406775..2407509 (-) | 735 | WP_004279229.1 | 3-oxoacyl-ACP reductase FabG | - |
| ABWED_RS11695 (ABWED_2433) | fabD | 2407506..2408492 (-) | 987 | WP_004730620.1 | ACP S-malonyltransferase | - |
| ABWED_RS11700 (ABWED_2434) | rpmF | 2408716..2408898 (-) | 183 | WP_004279227.1 | 50S ribosomal protein L32 | - |
| ABWED_RS11705 (ABWED_2435) | - | 2408976..2409533 (-) | 558 | WP_004279226.1 | DUF177 domain-containing protein | - |
Sequence
Protein
Download Length: 373 a.a. Molecular weight: 41684.95 Da Isoelectric Point: 6.9359
>NTDB_id=599493 ABWED_RS11485 WP_004279277.1 2366399..2367520(+) (pilU) [Acinetobacter lwoffii strain ED23-35]
MDFNGLLDYMVEKKASDLFITADVEPSIKINGQILPIGSVKLPGAAVGQLLHSIMTEKQRKEFADSRECNFAISTPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLVGYRNENSKGHIITIE
DPIEFIHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGSHLLRAHSNITFDGQS
MDFNGLLDYMVEKKASDLFITADVEPSIKINGQILPIGSVKLPGAAVGQLLHSIMTEKQRKEFADSRECNFAISTPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLVGYRNENSKGHIITIE
DPIEFIHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGSHLLRAHSNITFDGQS
Nucleotide
Download Length: 1122 bp
>NTDB_id=599493 ABWED_RS11485 WP_004279277.1 2366399..2367520(+) (pilU) [Acinetobacter lwoffii strain ED23-35]
ATGGATTTTAACGGCTTACTCGATTATATGGTGGAGAAAAAAGCATCGGATCTGTTCATTACTGCCGATGTCGAACCGTC
CATCAAGATTAATGGGCAGATTTTACCGATTGGTTCGGTAAAATTACCCGGTGCGGCAGTAGGACAACTGCTCCATTCCA
TCATGACCGAAAAGCAGCGTAAAGAATTTGCTGACAGTCGTGAATGTAACTTTGCCATCAGCACGCCGGATAAAAGTGCC
CGCTTCCGTGTCAGTGCCTTTCAGCAACGAGATCAACCGGGGATGGTGCTGCGTCGGATTGAAACTGTTATTCCCACGAT
GGATGAACTCAAGTTACCGCCGATTCTAAAAGAACTGGCGATGACCAAACGTGGCATCATTATTTTTGTCGGGGCAACGG
GTACCGGTAAATCAACCTCGCTGGCGTCTTTGGTCGGTTATCGTAATGAAAATTCCAAAGGTCATATCATTACCATTGAA
GATCCGATCGAATTTATTCACCAGCATAAGGGCTGTATTATCACCCAGCGTGAAGTCGGCATCGATACTGATTCATTTGA
AATTGCCCTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCACGTGAAACCATGGATT
ATGCGATTGCCTTTGCCGAAACCGGTCATCTGGTCTTTGCTACCCTGCACGCCAACAACGCCAACCAAGCGATTGACCGG
ATTATCCATTTCTTCGAAGCAGACCGACATAATCAGCTGTTCATGGACTTGTCCCTGAACATGAAAGCCATTGTGGCACA
GCAACTGATTCCAACCATCGATGGTAATGGTCGCCGTGCAGCGATTGAGATTCTGATTAACTCACCACTGATTGCCGACT
TGATTCGTAAAGGTGATGTGCATGAGATCAAGGATCTGATGAAGCGTTCCCGTGAACTGGGCATGCAGACCTTCGATCAG
GCGCTGTATGATCTGTATAAAGCCAAACAGATCAGCTATAAAGATGCACTGAAACATGCAGATTCACCAAACGACCTGCG
TCTGCAAATCAAGCTGTCAGAAGAAGGTGGTAGTCACTTATTACGTGCGCATTCCAACATTACCTTTGATGGTCAGTCCT
GA
ATGGATTTTAACGGCTTACTCGATTATATGGTGGAGAAAAAAGCATCGGATCTGTTCATTACTGCCGATGTCGAACCGTC
CATCAAGATTAATGGGCAGATTTTACCGATTGGTTCGGTAAAATTACCCGGTGCGGCAGTAGGACAACTGCTCCATTCCA
TCATGACCGAAAAGCAGCGTAAAGAATTTGCTGACAGTCGTGAATGTAACTTTGCCATCAGCACGCCGGATAAAAGTGCC
CGCTTCCGTGTCAGTGCCTTTCAGCAACGAGATCAACCGGGGATGGTGCTGCGTCGGATTGAAACTGTTATTCCCACGAT
GGATGAACTCAAGTTACCGCCGATTCTAAAAGAACTGGCGATGACCAAACGTGGCATCATTATTTTTGTCGGGGCAACGG
GTACCGGTAAATCAACCTCGCTGGCGTCTTTGGTCGGTTATCGTAATGAAAATTCCAAAGGTCATATCATTACCATTGAA
GATCCGATCGAATTTATTCACCAGCATAAGGGCTGTATTATCACCCAGCGTGAAGTCGGCATCGATACTGATTCATTTGA
AATTGCCCTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCACGTGAAACCATGGATT
ATGCGATTGCCTTTGCCGAAACCGGTCATCTGGTCTTTGCTACCCTGCACGCCAACAACGCCAACCAAGCGATTGACCGG
ATTATCCATTTCTTCGAAGCAGACCGACATAATCAGCTGTTCATGGACTTGTCCCTGAACATGAAAGCCATTGTGGCACA
GCAACTGATTCCAACCATCGATGGTAATGGTCGCCGTGCAGCGATTGAGATTCTGATTAACTCACCACTGATTGCCGACT
TGATTCGTAAAGGTGATGTGCATGAGATCAAGGATCTGATGAAGCGTTCCCGTGAACTGGGCATGCAGACCTTCGATCAG
GCGCTGTATGATCTGTATAAAGCCAAACAGATCAGCTATAAAGATGCACTGAAACATGCAGATTCACCAAACGACCTGCG
TCTGCAAATCAAGCTGTCAGAAGAAGGTGGTAGTCACTTATTACGTGCGCATTCCAACATTACCTTTGATGGTCAGTCCT
GA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Acinetobacter baylyi ADP1 |
72.222 |
100 |
0.732 |
| pilU | Pseudomonas stutzeri DSM 10701 |
69.003 |
99.464 |
0.686 |
| pilU | Vibrio cholerae strain A1552 |
57.55 |
94.102 |
0.542 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
42.486 |
92.761 |
0.394 |
| pilT | Acinetobacter baumannii strain A118 |
41.691 |
91.957 |
0.383 |
| pilT | Acinetobacter nosocomialis M2 |
41.691 |
91.957 |
0.383 |
| pilT | Acinetobacter baumannii D1279779 |
41.691 |
91.957 |
0.383 |
| pilT | Legionella pneumophila strain Lp02 |
42.262 |
90.08 |
0.381 |
| pilT | Legionella pneumophila strain ERS1305867 |
42.262 |
90.08 |
0.381 |
| pilT | Pseudomonas aeruginosa PAK |
40.462 |
92.761 |
0.375 |
| pilT | Vibrio cholerae strain A1552 |
39.601 |
94.102 |
0.373 |
| pilT | Vibrio cholerae O1 biovar El Tor strain E7946 |
39.601 |
94.102 |
0.373 |
| pilT | Acinetobacter baylyi ADP1 |
40.233 |
91.957 |
0.37 |
| pilT | Neisseria gonorrhoeae MS11 |
39.595 |
92.761 |
0.367 |
| pilT | Neisseria meningitidis 8013 |
39.595 |
92.761 |
0.367 |
| pilT | Pseudomonas stutzeri DSM 10701 |
39.017 |
92.761 |
0.362 |