Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NBY25_RS20445 Genome accession   NZ_CP098187
Coordinates   4211719..4213710 (-) Length   663 a.a.
NCBI ID   WP_000098601.1    Uniprot ID   A0A0H3EL60
Organism   Escherichia coli strain Z0117EC0117     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4206719..4218710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY25_RS20420 (NBY25_20415) - 4206752..4207067 (-) 316 Protein_3994 fructose-bisphosphatase class II -
  NBY25_RS20425 (NBY25_20420) yggP 4207064..4208341 (-) 1278 WP_000853182.1 zinc-binding dehydrogenase -
  NBY25_RS20430 (NBY25_20425) cmtA 4208356..4209744 (-) 1389 WP_000428786.1 PTS mannitol transporter subunit IICB -
  NBY25_RS20435 (NBY25_20430) cmtB 4209772..4210215 (-) 444 WP_001236690.1 PTS mannitol transporter subunit IIA -
  NBY25_RS20440 (NBY25_20435) - 4210592..4211500 (+) 909 WP_000646942.1 prohibitin family protein -
  NBY25_RS20445 (NBY25_20440) recP/tkt 4211719..4213710 (-) 1992 WP_000098601.1 transketolase Machinery gene
  NBY25_RS20450 (NBY25_20445) loiP 4213988..4214746 (+) 759 WP_001327406.1 metalloprotease LoiP -
  NBY25_RS20455 (NBY25_20450) speB 4214950..4215870 (-) 921 WP_000105562.1 agmatinase -
  NBY25_RS20460 (NBY25_20455) - 4216006..4216737 (-) 732 WP_000758888.1 hypothetical protein -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72182.71 Da        Isoelectric Point: 5.5018

>NTDB_id=597523 NBY25_RS20445 WP_000098601.1 4211719..4213710(-) (recP/tkt) [Escherichia coli strain Z0117EC0117]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQIA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AEQLFEEFGFTVDNVVAKAKALL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=597523 NBY25_RS20445 WP_000098601.1 4211719..4213710(-) (recP/tkt) [Escherichia coli strain Z0117EC0117]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCACCCGGAAGTGGGTTACACCGC
AGGTGTAGAAACCACCACCGGTCCGCTGGGTCAGGGTATTGCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATCGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCGTTCTACGATGACAACGGTAT
TTCTATCGATGGTCACGTTGAAGGCTGGTTTACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGATATCGACGGTCATGACGCGGCATCCATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCGTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACCCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAGCTGGGCTGGAAATACGCGCCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAATCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCCAAAGCGAAAGAGTTCATCGCTAA
ACTTCAGGCTAATCCGGCGAAGATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCGTTCGGGCCGCTGTTGCCGG
AATTCCTCGGCGGCTCCGCTGACCTGGCGCCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTACGTGAATTCGGTATGACTGCGATTGCCAACGGTATCTCCCTGCACGGTGGTTT
CCTGCCGTATACCTCAACCTTCCTGATGTTCGTGGAATACGCACGTAATGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGATGGCCCGACTCACCAGCCGGTAGAACAGATCGCT
TCTCTGCGCGTGACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCAGTAGCGTGGAAATACGGCGT
TGAGCGTCAGGACGGCCCGACTGCGCTTATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGTACTGAAGAGCAGCTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGTGCTGGTCAGCCGGAACTGATCTTCATCGCGACCGGTTCTGAA
GTTGAACTGGCTGTTGCCGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTTTCCATGCCGTCTACCGA
CGCATTCGACAAGCAGGACGCGGCTTATCGTGAATCCGTACTGCCGAAAGCGGTTACCGCTCGTGTTGCGGTGGAAGCTG
GCATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGCATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCAGCTGTTTGAAGAGTTCGGCTTCACCGTTGACAACGTTGTTGCGAAAGCAAAAGCGCTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EL60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.024

99.246

0.477