Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   M8Z32_RS23865 Genome accession   NZ_CP097555
Coordinates   5134402..5134869 (-) Length   155 a.a.
NCBI ID   WP_003161762.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain B1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5129402..5139869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8Z32_RS23850 (M8Z32_23825) nadC 5131902..5132750 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M8Z32_RS23860 (M8Z32_23835) - 5132931..5134244 (-) 1314 WP_223218619.1 O-antigen ligase family protein -
  M8Z32_RS23865 (M8Z32_23840) pilA/pilAI 5134402..5134869 (-) 468 WP_003161762.1 pilin Machinery gene
  M8Z32_RS23870 (M8Z32_23845) pilB 5135100..5136800 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M8Z32_RS23875 (M8Z32_23850) pilC 5136804..5138021 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  M8Z32_RS23880 (M8Z32_23855) pilD 5138022..5138894 (+) 873 WP_003161765.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M8Z32_RS23885 (M8Z32_23860) coaE 5138891..5139502 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  M8Z32_RS23890 (M8Z32_23865) yacG 5139499..5139699 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15959.30 Da        Isoelectric Point: 9.0145

>NTDB_id=595140 M8Z32_RS23865 WP_003161762.1 5134402..5134869(-) (pilA/pilAI) [Pseudomonas aeruginosa strain B1.2]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=595140 M8Z32_RS23865 WP_003161762.1 5134402..5134869(-) (pilA/pilAI) [Pseudomonas aeruginosa strain B1.2]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GTGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.679

100

0.477
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.439
  pilA Acinetobacter baumannii strain A118

43.312

100

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

37.427

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.262

90.968

0.394
  pilA Vibrio cholerae strain A1552

43.262

90.968

0.394
  pilA Vibrio cholerae C6706

43.262

90.968

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.333

96.774

0.381
  pilA/pilA1 Eikenella corrodens VA1

38.095

94.839

0.361