Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M4R69_RS21545 Genome accession   NZ_CP097198
Coordinates   4456540..4458033 (+) Length   497 a.a.
NCBI ID   WP_001530857.1    Uniprot ID   -
Organism   Escherichia coli strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4451540..4463033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4R69_RS21525 (M4R69_21520) pilD 4452188..4452997 (+) 810 WP_001697419.1 prepilin peptidase PppA Machinery gene
  M4R69_RS21530 (M4R69_21525) gspS2 4453063..4453473 (+) 411 WP_001361238.1 type II secretion system pilot lipoprotein GspS-beta -
  M4R69_RS21535 (M4R69_21530) gspC 4453491..4454450 (+) 960 WP_000135085.1 type II secretion system protein GspC -
  M4R69_RS21540 (M4R69_21535) gspD 4454480..4456540 (+) 2061 WP_001468189.1 type II secretion system secretin GspD -
  M4R69_RS21545 (M4R69_21540) pilB/pilB1 4456540..4458033 (+) 1494 WP_001530857.1 type II secretion system ATPase GspE Machinery gene
  M4R69_RS21550 (M4R69_21545) gspF 4458033..4459256 (+) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  M4R69_RS21555 (M4R69_21550) gspG 4459273..4459728 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  M4R69_RS21560 (M4R69_21555) gspH 4459732..4460295 (+) 564 WP_001115117.1 type II secretion system minor pseudopilin GspH -
  M4R69_RS21565 (M4R69_21560) gspI 4460292..4460663 (+) 372 WP_000820092.1 type II secretion system minor pseudopilin GspI -
  M4R69_RS21570 (M4R69_21565) gspJ 4460660..4461265 (+) 606 WP_001254778.1 type II secretion system minor pseudopilin GspJ -
  M4R69_RS21575 (M4R69_21570) gspK 4461262..4462239 (+) 978 WP_001598627.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55180.33 Da        Isoelectric Point: 5.9081

>NTDB_id=593969 M4R69_RS21545 WP_001530857.1 4456540..4458033(+) (pilB/pilB1) [Escherichia coli strain 2-1]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=593969 M4R69_RS21545 WP_001530857.1 4456540..4458033(+) (pilB/pilB1) [Escherichia coli strain 2-1]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTATAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCCTCC
GAGGCTCGCCAGTTGATGGAAGATCTCGGCTCCGCCGAGGACTTTTTTACCCTCGCAGAAGAGTTACCGGAAACCGAGGA
TTTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362