Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MX621_RS28015 Genome accession   NZ_CP095923
Coordinates   5969343..5969966 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain AR19438     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5964343..5974966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX621_RS27995 (MX621_27995) aroB 5964938..5966044 (-) 1107 WP_043091242.1 3-dehydroquinate synthase -
  MX621_RS28000 (MX621_28000) aroK 5966094..5966612 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MX621_RS28005 (MX621_28005) pilQ 5966624..5968768 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  MX621_RS28010 (MX621_28010) comL/pilP 5968822..5969346 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  MX621_RS28015 (MX621_28015) pilO 5969343..5969966 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  MX621_RS28020 (MX621_28020) pilN 5969963..5970559 (-) 597 WP_043091243.1 type 4a pilus biogenesis protein PilN Machinery gene
  MX621_RS28025 (MX621_28025) pilM 5970559..5971623 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  MX621_RS28030 (MX621_28030) - 5971808..5974279 (+) 2472 WP_003110889.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=585696 MX621_RS28015 WP_003103268.1 5969343..5969966(-) (pilO) [Pseudomonas aeruginosa strain AR19438]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=585696 MX621_RS28015 WP_003103268.1 5969343..5969966(-) (pilO) [Pseudomonas aeruginosa strain AR19438]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAACTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401