Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   B1NLA3E_RS20535 Genome accession   NC_021171
Coordinates   4269408..4270571 (-) Length   387 a.a.
NCBI ID   WP_015595739.1    Uniprot ID   N0AYE3
Organism   Bacillus sp. 1NLA3E     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264408..4275571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1NLA3E_RS20510 (B1NLA3E_19745) prfB 4264705..4265806 (+) 1102 WP_144061517.1 peptide chain release factor 2 -
  B1NLA3E_RS20515 (B1NLA3E_19750) - 4266175..4266705 (-) 531 WP_015595735.1 chromate transporter -
  B1NLA3E_RS20520 (B1NLA3E_19755) - 4266705..4267274 (-) 570 WP_015595736.1 chromate transporter -
  B1NLA3E_RS20525 (B1NLA3E_19760) raiA 4267588..4268139 (-) 552 WP_015595737.1 ribosome-associated translation inhibitor RaiA -
  B1NLA3E_RS20530 (B1NLA3E_19765) - 4268630..4269355 (-) 726 WP_015595738.1 ComF family protein -
  B1NLA3E_RS20535 (B1NLA3E_19770) comFA 4269408..4270571 (-) 1164 WP_015595739.1 DEAD/DEAH box helicase Machinery gene
  B1NLA3E_RS24465 - 4271074..4273632 (-) 2559 WP_236619590.1 SH3 domain-containing protein -
  B1NLA3E_RS20545 (B1NLA3E_19785) - 4273926..4274774 (-) 849 WP_015595742.1 DegV family protein -
  B1NLA3E_RS24470 (B1NLA3E_19790) sda 4274949..4275089 (-) 141 WP_015595743.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43487.39 Da        Isoelectric Point: 8.5902

>NTDB_id=58415 B1NLA3E_RS20535 WP_015595739.1 4269408..4270571(-) (comFA) [Bacillus sp. 1NLA3E]
MLISWRSESVPELVGANETTALLALHEAKMADQSSLLAWTGRLSEGQQLASNRVIEAIQQKDELLVWAVCGAGKTEVLFA
GIDRALQQGQRVCIATPRTDVVLELTPRLQAAFPTISIASLYGGSPDRHLCAPLTITTTHQLLRFYQAFDTVIVDEVDAF
PYSVDETLQYAVKQSRKPKSGLIYLTATPDQKLQRQCRNGKKAYTTIPARFHRHPLPVPEFVWCGNWGKLLKGKQTLPQN
VMKWIDERLKIHKQALLFIPKIELIDELLPLLQAKYPTIEGVHAEDPDRKEKVMKMRNHEIPLLLTTTILERGVTFPNID
VAVLGAEDRIFTESALVQISGRVGRSPDFPSGDITFFHYGKTEAMVKARKQIIDMNREAKRVGLVDF

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=58415 B1NLA3E_RS20535 WP_015595739.1 4269408..4270571(-) (comFA) [Bacillus sp. 1NLA3E]
GTGCTGATAAGCTGGCGTTCAGAATCGGTACCTGAACTGGTTGGTGCAAATGAGACCACAGCTTTGTTAGCTCTTCATGA
GGCGAAAATGGCAGACCAATCAAGCTTATTAGCTTGGACCGGAAGGCTATCTGAAGGCCAACAGTTGGCCTCAAATCGGG
TGATAGAGGCCATACAACAAAAGGATGAGCTCTTGGTTTGGGCGGTTTGTGGGGCTGGCAAAACCGAGGTGCTGTTTGCT
GGGATTGACCGCGCACTCCAGCAGGGCCAAAGAGTGTGTATCGCCACGCCTCGGACCGATGTTGTACTCGAGCTCACTCC
ACGACTGCAAGCAGCCTTCCCGACGATTTCAATAGCATCCTTATATGGTGGTAGCCCAGACCGCCATTTGTGCGCTCCGC
TAACCATAACCACCACTCATCAGCTTCTCCGCTTTTATCAAGCCTTTGACACGGTCATCGTTGATGAGGTAGATGCATTC
CCATATTCTGTTGATGAAACCCTTCAGTATGCCGTAAAACAATCAAGAAAGCCTAAATCTGGGCTTATCTATTTAACGGC
GACCCCAGATCAAAAGCTGCAGAGGCAATGTCGTAATGGAAAAAAAGCGTACACCACCATCCCGGCCCGTTTTCATCGTC
ATCCACTGCCTGTGCCGGAGTTCGTTTGGTGTGGGAATTGGGGGAAGTTGTTAAAAGGTAAACAGACTCTTCCCCAAAAT
GTGATGAAGTGGATCGATGAGCGATTGAAAATCCACAAGCAAGCCCTTTTGTTCATTCCAAAAATCGAGCTTATTGACGA
GCTACTGCCCCTCCTTCAGGCGAAGTATCCCACAATCGAAGGCGTTCATGCCGAGGACCCGGACCGTAAGGAAAAGGTTA
TGAAGATGCGCAACCACGAAATCCCTCTGTTACTTACCACAACTATCCTTGAAAGAGGCGTGACCTTCCCAAATATTGAT
GTTGCCGTCCTTGGAGCCGAGGACCGCATTTTTACCGAGAGTGCTCTCGTGCAAATTTCTGGCCGGGTTGGACGTAGTCC
TGACTTCCCATCGGGTGATATCACTTTTTTTCATTATGGAAAAACGGAGGCGATGGTCAAAGCCCGCAAACAAATTATAG
ACATGAACCGGGAGGCAAAGCGAGTTGGACTTGTTGATTTTTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(43-190)

Helicase conserved C-terminal domain (Helicase_C)

(248-345)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N0AYE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.83

95.866

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

45.402

89.922

0.408
  comFA/cflA Streptococcus mitis SK321

43.266

90.181

0.39
  comFA/cflA Streptococcus pneumoniae Rx1

42.407

90.181

0.382
  comFA/cflA Streptococcus pneumoniae D39

42.407

90.181

0.382
  comFA/cflA Streptococcus pneumoniae R6

42.407

90.181

0.382
  comFA/cflA Streptococcus mitis NCTC 12261

42.407

90.181

0.382
  comFA/cflA Streptococcus pneumoniae TIGR4

42.12

90.181

0.38
  comFA Lactococcus lactis subsp. cremoris KW2

40.346

89.664

0.362

Multiple sequence alignment