Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HU749_RS28120 Genome accession   NZ_CP077086
Coordinates   6347909..6348943 (-) Length   344 a.a.
NCBI ID   WP_014340671.1    Uniprot ID   A0A0F4XQQ3
Organism   Pseudomonas ogarae strain SWRI108     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6342909..6353943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU749_RS28090 (HU749_028090) ruvX 6343032..6343469 (+) 438 WP_003177497.1 Holliday junction resolvase RuvX -
  HU749_RS28095 (HU749_028095) pyrR 6343554..6344060 (+) 507 WP_014340669.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HU749_RS28100 (HU749_028100) - 6344085..6345089 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  HU749_RS28105 (HU749_028105) - 6345086..6346357 (+) 1272 WP_186649261.1 dihydroorotase -
  HU749_RS28110 (HU749_028110) - 6346491..6346919 (-) 429 WP_186649263.1 NINE protein -
  HU749_RS28115 (HU749_028115) - 6347169..6347780 (+) 612 WP_186649264.1 C40 family peptidase -
  HU749_RS28120 (HU749_028120) pilT 6347909..6348943 (-) 1035 WP_014340671.1 type IV pilus twitching motility protein PilT Machinery gene
  HU749_RS28125 (HU749_028125) - 6348997..6349683 (+) 687 WP_046063240.1 YggS family pyridoxal phosphate-dependent enzyme -
  HU749_RS28130 (HU749_028130) proC 6349719..6350537 (+) 819 WP_186649266.1 pyrroline-5-carboxylate reductase -
  HU749_RS28135 (HU749_028135) - 6350548..6351138 (+) 591 WP_014340674.1 YggT family protein -
  HU749_RS28140 (HU749_028140) - 6351143..6351436 (+) 294 WP_046063238.1 DUF167 domain-containing protein -
  HU749_RS28145 (HU749_028145) - 6351683..6352822 (+) 1140 WP_095962437.1 homoserine O-succinyltransferase MetX -
  HU749_RS28150 (HU749_028150) metW 6352830..6353450 (+) 621 WP_014340677.1 methionine biosynthesis protein MetW -
  HU749_RS28155 (HU749_028155) - 6353476..6353910 (+) 435 WP_014340678.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38137.75 Da        Isoelectric Point: 6.9777

>NTDB_id=578979 HU749_RS28120 WP_014340671.1 6347909..6348943(-) (pilT) [Pseudomonas ogarae strain SWRI108]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTESPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVSKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=578979 HU749_RS28120 WP_014340671.1 6347909..6348943(-) (pilT) [Pseudomonas ogarae strain SWRI108]
ATGGATATCACTGAGTTACTGGCGTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCACCGAT
GATCCGCGTCGATGGCGATGTCAGGCGCATCAACCTGCCGGCACTGGACCACAAGCAGGTTCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGAGTAGATTACGAAAAGTTTCTGGAGACCGATTTTTCCTTCGACGTGCCGGGCGTGGCGCGG
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGCGCAGGGGCGGTGTTCCGCACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAGGTGACCGAATCGCCGCGCGGGCTGGTGTTGGTGACCGGGCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCCATGATCGACTACTTGAACAGCCACAAGCACCATCACATCCTCACCATCGAAGAC
CCTATCGAATTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGTGAGGTGCACCGCGACACCCAGGGTTTCTCCAC
GGCCCTGCGTTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCTGCGGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCGGCCAAGACCATTGACCGGGTG
GTGGACGTGTTCCCCGGGGATGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGATTTCCCAGAC
GCTGGTCAAGAAAATCGGCGGCGGCCGGATCGCGGCGCATGAAATCATGCTGGGCACCTCGGCTATTCGGAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGTTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGACTTGGTGAGCAAGGGGCTGATCAGCCGCGAGCATGCGCGGGAGCGGGCGCGTACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F4XQQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.42

100

0.395