Detailed information NTG0575355

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KMZ16_RS12855
• Genome accession: NZ_CP076445
• Coordinates: 2495496..2496113 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis strain ps4100
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2490496..2501113

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KMZ16_RS12825 (KMZ16_12725)	rpsT	2490805..2491071 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KMZ16_RS12830 (KMZ16_12730)	holA	2491086..2492129 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
KMZ16_RS12835 (KMZ16_12735)	-	2492169..2492318 (-)	150	WP_003229985.1	hypothetical protein	-
KMZ16_RS12840 (KMZ16_12740)	yqzM	2492359..2492493 (+)	135	WP_003229983.1	YqzM family protein	-
KMZ16_RS12845 (KMZ16_12745)	comEC	2492533..2494863 (-)	2331	WP_080477815.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KMZ16_RS12850 (KMZ16_12750)	comEB	2494860..2495429 (-)	570	WP_015714288.1	ComE operon protein 2	-
KMZ16_RS12855 (KMZ16_12755)	comEA	2495496..2496113 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KMZ16_RS12860 (KMZ16_12760)	comER	2496197..2497018 (+)	822	WP_004398597.1	late competence protein ComER	-
KMZ16_RS12865 (KMZ16_12765)	yqeM	2497084..2497827 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KMZ16_RS12870 (KMZ16_12770)	rsfS	2497824..2498180 (-)	357	WP_003229971.1	ribosome silencing factor	-
KMZ16_RS12875 (KMZ16_12775)	yqeK	2498198..2498758 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KMZ16_RS12880 (KMZ16_12780)	nadD	2498748..2499317 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KMZ16_RS12885 (KMZ16_12785)	yhbY	2499329..2499619 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KMZ16_RS12890 (KMZ16_12790)	aroE	2499613..2500455 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=575355 KMZ16_RS12855 WP_004398514.1 2495496..2496113(-) (comEA) [Bacillus subtilis subsp. subtilis strain ps4100]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=575355 KMZ16_RS12855 WP_004398514.1 2495496..2496113(-) (comEA) [Bacillus subtilis subsp. subtilis strain ps4100]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39