Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MNG38_RS13175 Genome accession   NZ_CP093289
Coordinates   2552089..2552499 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain H2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2547089..2557499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNG38_RS13145 (MNG38_13145) - 2547176..2547316 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MNG38_RS13150 (MNG38_13150) yqeF 2547622..2548353 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  MNG38_RS13155 (MNG38_13155) cwlH 2548605..2549357 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MNG38_RS13160 (MNG38_13160) yqeD 2549544..2550170 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  MNG38_RS13165 (MNG38_13165) gnd 2550189..2551082 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MNG38_RS13170 (MNG38_13170) yqeB 2551334..2552056 (+) 723 WP_010886572.1 hypothetical protein -
  MNG38_RS13175 (MNG38_13175) nucA/comI 2552089..2552499 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MNG38_RS13180 (MNG38_13180) - 2552695..2553045 (+) 351 Protein_2554 sigma-70 family RNA polymerase sigma factor -
  MNG38_RS13185 (MNG38_13185) spoIVCA 2553073..2554533 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MNG38_RS13190 (MNG38_13190) - 2554491..2554669 (-) 179 Protein_2556 hypothetical protein -
  MNG38_RS13195 (MNG38_13195) arsC 2555024..2555443 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MNG38_RS13200 (MNG38_13200) acr3 2555455..2556495 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  MNG38_RS13205 (MNG38_13205) arsK 2556518..2556958 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MNG38_RS13210 (MNG38_13210) arsR 2557017..2557334 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=570937 MNG38_RS13175 WP_009967785.1 2552089..2552499(-) (nucA/comI) [Bacillus subtilis strain H2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=570937 MNG38_RS13175 WP_009967785.1 2552089..2552499(-) (nucA/comI) [Bacillus subtilis strain H2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529