NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	MM141_RS23810	Genome accession	NZ_CP093012
Coordinates	5111133..5111585 (-)	Length	150 a.a.
NCBI ID	WP_024947914.1	Uniprot ID	A4GRK7
Organism	Pseudomonas aeruginosa strain H20
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5106133..5116585

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MM141_RS23790 (MM141_23800)	ampD	5106839..5107405 (-)	567	WP_009875882.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
MM141_RS23795 (MM141_23805)	-	5107556..5109811 (-)	2256	WP_009875881.1	DUF1631 domain-containing protein	-
MM141_RS23800 (MM141_23810)	nadC	5110097..5110945 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
MM141_RS23810 (MM141_23820)	pilA	5111133..5111585 (-)	453	WP_024947914.1	pilin	Machinery gene
MM141_RS23815 (MM141_23825)	pilB	5111814..5113514 (+)	1701	WP_009875880.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MM141_RS23820 (MM141_23830)	pilC	5113518..5114738 (+)	1221	WP_016253888.1	type 4a pilus biogenesis protein PilC	Machinery gene
MM141_RS23825 (MM141_23835)	pilD	5114742..5115614 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
MM141_RS23830 (MM141_23840)	coaE	5115611..5116222 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
MM141_RS23835 (MM141_23845)	yacG	5116219..5116419 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15622.02 Da Isoelectric Point: 8.4827

                                >NTDB_id=569190 MM141_RS23810 WP_024947914.1 5111133..5111585(-) (pilA) [Pseudomonas aeruginosa strain H20]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG

VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide

Download Length: 453 bp

                                >NTDB_id=569190 MM141_RS23810 WP_024947914.1 5111133..5111585(-) (pilA) [Pseudomonas aeruginosa strain H20]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT

TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG

AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG

GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC

TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA

AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A4GRK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	100	100	1
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.682	100	0.48
pilA	Acinetobacter baumannii strain A118	45.333	100	0.453
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.575	97.333	0.453
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.675	100	0.447
pilA	Vibrio cholerae C6706	42.675	100	0.447
pilA	Vibrio cholerae strain A1552	42.675	100	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	48.507	89.333	0.433
comP	Acinetobacter baylyi ADP1	40.789	100	0.413
pilA2	Legionella pneumophila strain ERS1305867	39.865	98.667	0.393
pilA/pilA1	Eikenella corrodens VA1	39.456	98	0.387
pilA2	Legionella pneumophila str. Paris	39.189	98.667	0.387

Detailed information NTG0569190