Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AVFI_RS13010 Genome accession   NZ_CP092712
Coordinates   2829053..2830552 (-) Length   499 a.a.
NCBI ID   WP_188863871.1    Uniprot ID   -
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2824053..2835552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS12980 (AVFI_12980) gspK 2824642..2825676 (-) 1035 WP_188863839.1 type II secretion system minor pseudopilin GspK -
  AVFI_RS12985 (AVFI_12985) gspJ 2825676..2826350 (-) 675 WP_155662863.1 type II secretion system minor pseudopilin GspJ -
  AVFI_RS12990 (AVFI_12990) gspI 2826337..2826714 (-) 378 WP_054775824.1 type II secretion system minor pseudopilin GspI -
  AVFI_RS12995 (AVFI_12995) gspH 2826704..2827300 (-) 597 WP_063650031.1 type II secretion system minor pseudopilin GspH -
  AVFI_RS13000 (AVFI_13000) gspG 2827323..2827775 (-) 453 WP_011262830.1 type II secretion system major pseudopilin GspG -
  AVFI_RS13005 (AVFI_13005) gspF 2827838..2829052 (-) 1215 WP_065639789.1 type II secretion system inner membrane protein GspF -
  AVFI_RS13010 (AVFI_13010) pilB/pilB1 2829053..2830552 (-) 1500 WP_188863871.1 type II secretion system ATPase GspE Machinery gene
  AVFI_RS13015 (AVFI_13015) gspD 2830552..2832588 (-) 2037 WP_188863872.1 type II secretion system secretin GspD -
  AVFI_RS13020 (AVFI_13020) gspC 2832621..2833529 (-) 909 WP_188863873.1 type II secretion system protein GspC -
  AVFI_RS13025 (AVFI_13025) hslR 2833809..2834210 (+) 402 WP_069581975.1 ribosome-associated heat shock protein Hsp15 -
  AVFI_RS13030 (AVFI_13030) hslO 2834303..2835178 (+) 876 WP_005421430.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55317.38 Da        Isoelectric Point: 5.6706

>NTDB_id=567987 AVFI_RS13010 WP_188863871.1 2829053..2830552(-) (pilB/pilB1) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MADTLFPESNAVLRLLFSFAKRHSVVIESSVHGWVLYYVSPPSSMVLCEIRRVCQSSFSLIQLTKDEFDTKLTQVYQRNS
SEAQQLMEDIGADSDDFFSLAEELPNNDDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMSKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLNSLGMSAE
NHKNFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSSQSNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCGECKEAY
QADEEQKKLFSLSASDELTLYKPIGCEHCNGKGYRGRTGIHELLVVNEKVQELIHKEVGEQAIEKEVRKSTRSIQQDGLL
KVQKGITTLEEVMRVTREG

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=567987 AVFI_RS13010 WP_188863871.1 2829053..2830552(-) (pilB/pilB1) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGGCGGATACATTATTTCCTGAATCTAACGCTGTACTTCGTTTGCTTTTTTCATTTGCAAAAAGGCATTCGGTAGTCAT
TGAATCAAGTGTTCATGGATGGGTGCTGTACTATGTATCGCCCCCTTCATCTATGGTGCTGTGTGAGATCCGCCGTGTTT
GCCAATCTAGTTTTTCATTAATTCAGTTAACCAAAGATGAGTTCGATACTAAGTTAACTCAGGTATATCAACGTAACTCA
TCAGAAGCTCAACAGTTAATGGAAGATATCGGTGCAGACAGTGACGATTTCTTTTCATTAGCTGAAGAGTTACCAAATAA
TGATGATTTATTAGAATCAGAAGATGATGCGCCGATCATTAAATTAATCAATGCCATGCTAGGTGAAGCGATTAAAGAGG
GGGCATCGGATATTCATATTGAAACGTTTGAGAAAGTATTGTCGATTCGTTTCCGTGTCGATGGCGTGTTGCGTGATGTA
TTAAGCCCAAGCCGTAAGTTAGCGCCATTATTGGTGTCTCGTGTAAAAGTGATGTCGAAATTGGACATCGCTGAAAAACG
AGTCCCACAAGATGGTCGTATTTCATTGCGCATTGGTGGTCGCGCTGTTGATGTGCGTGTATCAACCATGCCGTCATCAC
ATGGTGAGCGCGTGGTAATGCGTTTGTTGGATAAGAACGCAACTCGACTGGATTTAAACAGTTTAGGCATGTCGGCTGAA
AACCATAAAAACTTTCAGCATATTATCAAGCGTCCACATGGCATTATTTTGGTTACTGGCCCAACCGGTTCTGGTAAATC
AACGACCTTGTATGCAGGGTTAGGTGAACTAGACAGCTCTCAATCCAATATTTTAACCGTTGAAGACCCTATCGAATTTG
ATATTGATGGCATTGGCCAAACACAAGTAAATCCAAAAGTAGACATGACGTTTGCCCGTGGTTTACGTGCCATTCTTCGT
CAAGATCCTGATGTGGTGATGATCGGTGAGATCCGTGATTTAGAAACCGCAGAAATTGCCGTTCAAGCTTCATTAACCGG
TCACTTGGTAATGTCTACACTGCACACCAATACTGCGGTAGGTGCGATTACTCGTCTTCGAGATATGGGCATTGAACCTT
TCCTAATTTCATCTTCACTACTTGGGGTGCTTGCACAACGTCTGGTTAGAACTCTGTGTGGTGAATGTAAAGAAGCGTAT
CAAGCTGATGAGGAGCAGAAGAAATTGTTCTCTCTTTCAGCAAGTGACGAGTTAACGCTATATAAACCAATTGGTTGTGA
GCACTGTAATGGTAAAGGCTATCGTGGTCGTACGGGTATTCATGAACTTTTAGTTGTGAATGAAAAAGTACAAGAGTTGA
TCCACAAAGAAGTGGGTGAACAAGCGATAGAAAAAGAAGTAAGAAAGTCGACTCGCAGTATTCAACAAGACGGCTTATTA
AAAGTACAAAAAGGAATAACCACATTAGAAGAGGTGATGCGAGTCACTCGGGAAGGATAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

43.825

100

0.441
  pilB Legionella pneumophila strain ERS1305867

44.057

97.796

0.431
  pilF Thermus thermophilus HB27

47.761

80.561

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

43.908

87.174

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.716

78.958

0.377
  pilB Vibrio cholerae strain A1552

47.475

79.359

0.377
  pilB Vibrio campbellii strain DS40M4

42.529

87.174

0.371
  pilB Acinetobacter baumannii D1279779

46.684

78.557

0.367
  pilB Acinetobacter baylyi ADP1

46.154

78.156

0.361