Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AVFI_RS12040 Genome accession   NZ_CP092712
Coordinates   2632837..2633403 (-) Length   188 a.a.
NCBI ID   WP_011262703.1    Uniprot ID   Q5E2F5
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2627837..2638403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS12015 (AVFI_12015) aroB 2628105..2629193 (-) 1089 WP_011262699.1 3-dehydroquinate synthase -
  AVFI_RS12020 (AVFI_12020) aroK 2629230..2629748 (-) 519 WP_005421091.1 shikimate kinase AroK -
  AVFI_RS12025 (AVFI_12025) pilQ 2629973..2631661 (-) 1689 WP_012533907.1 type IV pilus secretin PilQ Machinery gene
  AVFI_RS12030 (AVFI_12030) pilP 2631748..2632266 (-) 519 WP_005421095.1 pilus assembly protein PilP Machinery gene
  AVFI_RS12035 (AVFI_12035) pilO 2632259..2632837 (-) 579 WP_005421098.1 type 4a pilus biogenesis protein PilO Machinery gene
  AVFI_RS12040 (AVFI_12040) pilN 2632837..2633403 (-) 567 WP_011262703.1 PilN domain-containing protein Machinery gene
  AVFI_RS12045 (AVFI_12045) pilM 2633388..2634389 (-) 1002 WP_054775331.1 type IV pilus biogenesis protein PilM Machinery gene
  AVFI_RS12050 (AVFI_12050) - 2634528..2637026 (+) 2499 WP_026029395.1 penicillin-binding protein 1A -
  AVFI_RS12055 (AVFI_12055) oxyR 2637093..2637985 (-) 893 Protein_2310 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 188 a.a.        Molecular weight: 21742.03 Da        Isoelectric Point: 9.3705

>NTDB_id=567981 AVFI_RS12040 WP_011262703.1 2632837..2633403(-) (pilN) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MAYQINLLPWREKQRARYKQRFIALLCAGVTLSVGIQWGIAQYLEQQVSIQTQRKQSLEKHISWLNNELKDLNEVGKEHE
AILTRLDVVEQLQQNRNKTTQLLNILPDIITEGIYLNKVRMHEREVEIKGFSDSNSRLASMLDKLERSSQITSVEMHSII
SGQKIFGHDVSSFEVSFKLLPPVKKEPS

Nucleotide


Download         Length: 567 bp        

>NTDB_id=567981 AVFI_RS12040 WP_011262703.1 2632837..2633403(-) (pilN) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGGCTTACCAAATAAACCTACTACCTTGGCGAGAAAAGCAGAGAGCAAGATATAAGCAACGTTTTATTGCATTACTTTG
TGCAGGGGTGACGCTATCTGTCGGTATTCAATGGGGGATTGCGCAATATCTAGAGCAACAAGTTTCGATTCAGACACAAC
GTAAGCAATCGTTAGAAAAGCATATCTCTTGGTTAAACAATGAATTAAAAGATCTTAACGAAGTTGGAAAGGAGCATGAA
GCGATATTAACTCGTCTTGATGTTGTCGAACAATTACAGCAGAACCGAAACAAAACCACTCAGTTATTGAACATTTTACC
CGACATCATTACTGAAGGTATTTATTTAAATAAAGTACGCATGCATGAAAGGGAAGTTGAGATTAAGGGGTTCAGTGATA
GTAACTCTCGGTTAGCAAGCATGTTGGATAAATTAGAGCGTTCCTCTCAAATTACTTCAGTAGAAATGCACTCGATTATC
TCAGGGCAAAAAATATTTGGTCATGATGTCAGTAGTTTTGAAGTCTCGTTTAAATTACTTCCTCCAGTGAAAAAGGAACC
GTCGTAA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E2F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

47.541

97.34

0.463
  pilN Vibrio campbellii strain DS40M4

46.328

94.149

0.436