Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LU695_RS01175 Genome accession   NZ_CP092500
Coordinates   254804..256297 (+) Length   497 a.a.
NCBI ID   WP_001040629.1    Uniprot ID   -
Organism   Escherichia coli strain A30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 249804..261297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU695_RS01145 (LU695_01145) tusA 250051..250296 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  LU695_RS01150 (LU695_01150) zntA 250398..252596 (-) 2199 WP_258314078.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  LU695_RS01155 (LU695_01155) yhhN 252670..253296 (-) 627 WP_000964718.1 lysoplasmalogenase -
  LU695_RS01160 (LU695_01160) yhhM 253437..253796 (+) 360 WP_100287061.1 DUF2500 domain-containing protein -
  LU695_RS01165 (LU695_01165) yhhL 253799..254068 (-) 270 WP_001295207.1 DUF1145 family protein -
  LU695_RS01170 (LU695_01170) rsmD 254058..254654 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  LU695_RS01175 (LU695_01175) pilA 254804..256297 (+) 1494 WP_001040629.1 signal recognition particle-docking protein FtsY Machinery gene
  LU695_RS01180 (LU695_01180) ftsE 256300..256968 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  LU695_RS01185 (LU695_01185) ftsX 256961..258019 (+) 1059 WP_112918666.1 permease-like cell division protein FtsX -
  LU695_RS01190 (LU695_01190) rpoH 258264..259118 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  LU695_RS01195 (LU695_01195) livJ 259389..260492 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  LU695_RS01200 (LU695_01200) panM 260691..261074 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54544.36 Da        Isoelectric Point: 4.1560

>NTDB_id=565510 LU695_RS01175 WP_001040629.1 254804..256297(+) (pilA) [Escherichia coli strain A30]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=565510 LU695_RS01175 WP_001040629.1 254804..256297(+) (pilA) [Escherichia coli strain A30]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCACAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTATTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACCGCCGGACGTCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.901

81.489

0.39