Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MJJ13_RS01765 Genome accession   NZ_CP092449
Coordinates   375188..376426 (+) Length   412 a.a.
NCBI ID   WP_000815984.1    Uniprot ID   -
Organism   Escherichia coli isolate TKEC21-17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370188..381426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJJ13_RS01740 (MJJ13_01735) mrcA 370468..373020 (-) 2553 WP_032247284.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  MJJ13_RS01745 (MJJ13_01740) hofM 373140..373919 (+) 780 WP_032247285.1 DNA utilization protein HofM -
  MJJ13_RS01750 (MJJ13_01745) hofN 373919..374458 (+) 540 WP_001069320.1 DNA utilization protein HofN -
  MJJ13_RS01755 (MJJ13_01750) hofO 374442..374882 (+) 441 WP_001055768.1 DNA utilization protein HofO -
  MJJ13_RS01760 (MJJ13_01755) hofP 374872..375276 (+) 405 WP_001338740.1 DNA utilization protein HofP -
  MJJ13_RS01765 (MJJ13_01760) comE 375188..376426 (+) 1239 WP_000815984.1 DNA uptake porin HofQ Machinery gene
  MJJ13_RS01770 (MJJ13_01765) aroK 376827..377348 (+) 522 WP_000818618.1 shikimate kinase AroK -
  MJJ13_RS01775 (MJJ13_01770) aroB 377405..378493 (+) 1089 WP_000439849.1 3-dehydroquinate synthase -
  MJJ13_RS01780 (MJJ13_01775) damX 378585..379883 (+) 1299 WP_024249927.1 cell division protein DamX -
  MJJ13_RS01785 (MJJ13_01780) dam 379990..380826 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44643.18 Da        Isoelectric Point: 6.3183

>NTDB_id=565188 MJJ13_RS01765 WP_000815984.1 375188..376426(+) (comE) [Escherichia coli isolate TKEC21-17]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=565188 MJJ13_RS01765 WP_000815984.1 375188..376426(+) (comE) [Escherichia coli isolate TKEC21-17]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAAGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCTGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTTGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367