Detailed information NTG0056457

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PA8380_RS27845
• Genome accession: NZ_AP014839
• Coordinates: 6015517..6016581 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain 8380
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6010517..6021581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA8380_RS27820 (PA8380_57130)	aroK	6011064..6011582 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PA8380_RS27825 (PA8380_57140)	pilQ	6011594..6013726 (-)	2133	WP_003116137.1	type 4a pilus secretin PilQ	Machinery gene
PA8380_RS27830 (PA8380_57150)	comL/pilP	6013780..6014304 (-)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PA8380_RS27835 (PA8380_57160)	pilO	6014301..6014924 (-)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	Machinery gene
PA8380_RS27840 (PA8380_57170)	pilN	6014921..6015517 (-)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
PA8380_RS27845 (PA8380_57180)	pilM	6015517..6016581 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PA8380_RS27850 (PA8380_57190)	-	6016766..6019237 (+)	2472	WP_017148635.1	penicillin-binding protein 1A	-
PA8380_RS27855 (PA8380_57200)	-	6019342..6020610 (-)	1269	WP_004347579.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=56457 PA8380_RS27845 WP_003110888.1 6015517..6016581(-) (pilM) [Pseudomonas aeruginosa strain 8380]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=56457 PA8380_RS27845 WP_003110888.1 6015517..6016581(-) (pilM) [Pseudomonas aeruginosa strain 8380]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTTGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466