NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	KCG54_RS10150	Genome accession	NZ_CP073115
Coordinates	2112210..2112497 (-)	Length	95 a.a.
NCBI ID	WP_036472233.1	Uniprot ID	A0A4D7WS56
Organism	Neisseria subflava strain TT0077
Function	DNA binding (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2095302..2112497	2112210..2112497	within	0

Gene organization within MGE regions

Location: 2095302..2112497

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCG54_RS10085 (KCG54_10110)	-	2095302..2095724 (-)	423	WP_254324098.1	hypothetical protein	-
KCG54_RS10090 (KCG54_10115)	-	2095766..2097940 (-)	2175	WP_254324099.1	type VI secretion system Vgr family protein	-
KCG54_RS10095 (KCG54_10120)	tssH	2098086..2100902 (-)	2817	WP_254324100.1	type VI secretion system ATPase TssH	-
KCG54_RS10100 (KCG54_10125)	-	2100974..2101462 (-)	489	WP_049332188.1	Hcp family type VI secretion system effector	-
KCG54_RS10105 (KCG54_10130)	-	2101533..2103161 (-)	1629	WP_254324101.1	OmpA family protein	-
KCG54_RS10110 (KCG54_10135)	-	2103179..2103841 (-)	663	WP_101756361.1	DotU/TssL family secretion system protein	-
KCG54_RS10115 (KCG54_10140)	tssK	2103843..2105189 (-)	1347	WP_254324102.1	type VI secretion system baseplate subunit TssK	-
KCG54_RS10120 (KCG54_10145)	tssB	2105349..2105849 (+)	501	WP_049332195.1	type VI secretion system contractile sheath small subunit	-
KCG54_RS10125 (KCG54_10150)	tssC	2105888..2107423 (+)	1536	WP_128580714.1	type VI secretion system contractile sheath large subunit	-
KCG54_RS10130 (KCG54_10155)	-	2107476..2107739 (-)	264	WP_049332348.1	PAAR domain-containing protein	-
KCG54_RS10135 (KCG54_10160)	-	2107786..2108931 (-)	1146	WP_254324103.1	hypothetical protein	-
KCG54_RS10140 (KCG54_10165)	uvrB	2109281..2111308 (-)	2028	WP_254324104.1	excinuclease ABC subunit UvrB	-
KCG54_RS10145 (KCG54_10170)	comP	2111530..2112024 (-)	495	WP_254324105.1	type IV pilin protein	Machinery gene
KCG54_RS10150 (KCG54_10175)	comE	2112210..2112497 (-)	288	WP_036472233.1	helix-hairpin-helix domain-containing protein	Machinery gene

Sequence

Protein

Download Length: 95 a.a. Molecular weight: 9763.42 Da Isoelectric Point: 10.4923

                                >NTDB_id=558052 KCG54_RS10150 WP_036472233.1 2112210..2112497(-) (comE) [Neisseria subflava strain TT0077]

MKKFLFGAFAAVCAAFSLAAVNINTASSAELEALPGIGPAKAKSIVEYRQKNGAFKSVEELKNVKGIGDAVLNKLKAEAT

VSSAAPKAAQPAVKK

Nucleotide

Download Length: 288 bp

                                >NTDB_id=558052 KCG54_RS10150 WP_036472233.1 2112210..2112497(-) (comE) [Neisseria subflava strain TT0077]

ATGAAGAAATTTTTATTTGGTGCATTTGCCGCTGTTTGTGCAGCATTCTCTTTGGCCGCCGTGAACATCAATACCGCATC

TTCTGCCGAACTGGAGGCCTTGCCGGGTATCGGCCCGGCTAAGGCGAAATCGATTGTAGAATACCGCCAGAAGAACGGTG

CGTTCAAATCGGTGGAGGAGCTGAAAAACGTGAAGGGCATTGGTGATGCGGTGCTGAACAAGTTGAAGGCGGAGGCGACG

GTTTCTTCTGCCGCGCCTAAGGCTGCCCAGCCTGCCGTGAAAAAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(20-77)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A4D7WS56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Neisseria gonorrhoeae MS11	62.626	100	0.653
comE	Neisseria gonorrhoeae MS11	62.626	100	0.653
comE	Neisseria gonorrhoeae MS11	62.626	100	0.653
comE	Neisseria gonorrhoeae MS11	62.626	100	0.653
Cj0011c	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	54.321	85.263	0.463
comEA	Vibrio parahaemolyticus RIMD 2210633	44.186	90.526	0.4

Detailed information NTG0558052